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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AP1M1 Monoclonal antibody (68726-1-Ig)

    WB analysis using 68726-1-Ig (same clone as 68726-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68726-1-Ig (AP1M1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68726-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AP1M1 Monoclonal antibody (68726-1-Ig)

    WB analysis using 68726-1-Ig (same clone as 68726-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68726-1-Ig (AP1M1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68726-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using AP1M1 Monoclonal antibody (68726-1-Ig)

    WB analysis of human placenta using 68726-1-Ig (same clone as 68726-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68726-1-Ig (AP1M1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68726-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using AP1M1 Monoclonal antibody (68726-1-Ig)

    WB analysis of A375 using 68726-1-Ig (same clone as 68726-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 68726-1-Ig (AP1M1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68726-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AP1M1 Monoclonal antibody (68726-1-Ig)

    IF Staining of HeLa using 68726-1-Ig (same clone as 68726-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AP1M1 antibody (68726-1-Ig, Clone: 1E9A11 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68726-1-PBS in a different storage buffer formulation.

AP1M1 Monoclonal antibody, PBS Only

AP1M1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68726-1-PBS
Clone No.1E9A11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

AP-1 complex subunit mu-1, AP mu chain family member mu1A, AP 1 complex subunit mu 1, Adaptor-related protein complex 1 subunit mu-1, Adaptor protein complex AP-1 subunit mu-1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68726-1-PBS targets AP1M1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag25303

Product name: Recombinant human AP1M1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 164-423 aa of BC017469

Sequence: IKYRKNEVFLDVIESVNLLVSANGNVLRSEIVGSIKMRVFLSGMPELRLGLNDKVLFDNTGRGKSKSVELEDVKFHQCVRLSRFENDRTISFIPPDGEFELMSYRLNTHVKPLIWIESVIEKHSHSRIEYMIKAKSQFKRRSTANNVEIHIPVPNDADSPKFKTTVGSVKWVPENSEIVWSIKSFPGGKEYLMRAHFGLPSVEAEDKEGKPPISVKFEIPYFTTSGIQVRYLKIIEKSGYQALPWVRYITQNGDYQLRTQ

相同性解析による交差性が予測される生物種
Full Name adaptor-related protein complex 1, mu 1 subunit
Calculated molecular weight 423 aa, 49 kDa
Observed molecular weight 47-49 kDa
GenBank accession numberBC017469
Gene Symbol AP1M1
Gene ID (NCBI) 8907
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9BXS5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

AP1M1, also named as CLTNM, Mu-adaptin 1 and Clathrin coat assembly protein AP47, belongs to the adaptor complexes medium subunit family. It is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

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