Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, mouse skeletal muscle tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IF | See 2 publications below |
Product Information
13258-1-AP targets Gamma Adaptin in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3696 Product name: Recombinant human Gamma adaptin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 476-825 aa of BC036283 Sequence: VQVAAWCIGEYGDLLVSGQCEEEEPIQVTEDEVLDILESVLISNMSTSVTRGYALTAIMKLSTRFTCTVNRIKKVVSIYGSSIDVELQQRAVEYNALFKKYDHMRSALLERMPVMEKVTTNGPTEIVQTNGETEPAPLETKPPPSGPQPTSQANDLLDLLGGNDITPVIPTAPTSKPSSAGGELLDLLGDINLTGAPAAAPAPASVPQISQPPFLLDGLSSQPLFNDIAAGIPSITAYSKNGLKIEFTFERSNTNPSVTVITIQASNSTELDMTDFVFQAAVPKTFQLQLLSPSSSIVPAFNTGTITQVIKVLNPQKQQLRMRIKLTYNHKGSAMQDLAEVNNFPPQSWQ 相同性解析による交差性が予測される生物種 |
| Full Name | adaptor-related protein complex 1, gamma 1 subunit |
| Calculated molecular weight | 825 aa, 92 kDa |
| Observed molecular weight | 91 kDa |
| GenBank accession number | BC036283 |
| Gene Symbol | Gamma Adaptin |
| Gene ID (NCBI) | 164 |
| RRID | AB_2058209 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43747 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Gamma Adaptin antibody 13258-1-AP | Download protocol |
| WB protocol for Gamma Adaptin antibody 13258-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins. | ||
Proc Natl Acad Sci U S A An in vitro vesicle formation assay reveals cargo clients and factors that mediate vesicular trafficking. | ||
Front Cell Dev Biol Visualization of Protein Sorting at the Trans-Golgi Network and Endosomes Through Super-Resolution Imaging. | ||
Cells Five Inhibitory Receptors Display Distinct Vesicular Distributions in Murine T Cells | ||
Front Cell Neurosci MEA6 Deficiency Impairs Cerebellar Development and Motor Performance by Tethering Protein Trafficking. | ||
