Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 12542-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12542-1-AP
WB result of Annexin VI antibody (12542-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin VI transfected HeLa cells.
WB analysis of human brain using 12542-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 12542-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12542-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12542-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 12542-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12542-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse placenta using 12542-1-AP
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 12542-1-AP
IP result of anti-Annexin VI (IP:12542-1-AP, 4ug; Detection:12542-1-AP 1:2000) with HeLa cells lysate 1200 ug.
IP experiment of mouse kidney using 12542-1-AP
IP result of anti-Annexin VI (IP:12542-1-AP, 4ug; Detection:12542-1-AP 1:2000) with mouse kidney tissue lysate 3400 ug.
IHC staining of human colon using 12542-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 10x lens).
IHC staining of human colon using 12542-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 40x lens).
IHC staining of human colon using 12542-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12542-1-AP (Annexin VI antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 12542-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 10x lens).
IHC staining of human testis using 12542-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 40x lens).
IHC staining of human kidney using 12542-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 10x lens).
IHC staining of human kidney using 12542-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 40x lens).
IF Staining of HepG2 using 12542-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Annexin VI antibody (12542-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

Annexin VI Polyclonal antibody

Annexin VI Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12542-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Annexin A6, ANXA6, 67 kDa calelectrin, Annexin 6, Annexin-6

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (13)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, A549 cells, human brain tissue, Jurkat cells, mouse brain tissue, mouse kidney tissue, mouse placenta tissue
Positive IP detected in	HeLa cells, mouse kidney tissue
Positive IHC detected in	human colon tissue, human kidney tissue, human normal colon, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 13 publications below
IHC	See 3 publications below
IF	See 4 publications below
IP	See 1 publications below

Product Information

12542-1-AP targets Annexin VI in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3263 Product name: Recombinant human ANXA6 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 324-674 aa of BC017046 Sequence: DDDAAGQFFPEAAQVAYQMWELSAVARVELKGTVRPANDFNPDADAKALRKAMKGLGTDEDTIIDIITHRSNVQRQQIRQTFKSHFGRDLMTDLKSEISGDLARLILGLMMPPAHYDAKQLKKAMEGAGTDEKALIEILATRTNAEIRAINEAYKEDYHKSLEDALSSDTSGHFRRILISLATGHREEGGENLDQAREDAQVAAEILEIADTPSGDKTSLETRFMTILCTRSYPHLRRVFQEFIKMTNYDVEHTIKKEMSGDVRDAFVAIVQSVKNKPLFFADKLYKSMKGAGTDEKTLTRIMVSRSEIDLLNIRREFIEKYDKSLHQAIEGDTSGDFLKALLALCGGED 相同性解析による交差性が予測される生物種
Full Name	annexin A6
Calculated molecular weight	673 aa, 76 kDa
Observed molecular weight	67 kDa
GenBank accession number	BC017046
Gene Symbol	Annexin VI
Gene ID (NCBI)	309
RRID	AB_2057753
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P08133
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ANXA6, also called CBP68, or 67 kDa calelectrin, belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis.

Protocols

Product Specific Protocols
WB protocol for Annexin VI antibody 12542-1-AP	Download protocol
IHC protocol for Annexin VI antibody 12542-1-AP	Download protocol
IF protocol for Annexin VI antibody 12542-1-AP	Download protocol
IP protocol for Annexin VI antibody 12542-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Adv Sci (Weinh) Isolation and Comprehensive Analysis of Cochlear Tissue-Derived Small Extracellular Vesicles Authors - Pei Jiang View Article
mouse	WB	J Extracell Vesicles Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion-exchange method. Authors - Naohiro Seo View Article
human	WB	Oncotarget CD300A promotes tumor progression by PECAM1, ADCY7 and AKT pathway in acute myeloid leukemia. Authors - Xiaogang Sun View Article
rat	WB	Front Cell Dev Biol Dynamic Mitochondrial Proteome Under Polyamines Treatment in Cardiac Aging. Authors - Hao Zhang View Article
rat	WB,IF	Proteome Sci TMT-based proteomics analysis of the cerebral cortex of TauT knockout rats. Authors - Yiming Xia View Article
rat	WB	Proteome Sci A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis. Authors - Jia Xiaofang X View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 12542-1-AP

WB analysis of HeLa using 12542-1-AP

WB analysis of human brain using 12542-1-AP

WB analysis of mouse kidney using 12542-1-AP

WB analysis of A549 using 12542-1-AP

WB analysis of Jurkat using 12542-1-AP

WB analysis of mouse brain using 12542-1-AP

WB analysis of HeLa using 12542-1-AP

WB analysis of mouse placenta using 12542-1-AP

IP experiment of HeLa using 12542-1-AP

IP experiment of mouse kidney using 12542-1-AP

IHC staining of human colon using 12542-1-AP

IHC staining of human colon using 12542-1-AP

IHC staining of human colon using 12542-1-AP

IHC staining of human testis using 12542-1-AP

IHC staining of human testis using 12542-1-AP

IHC staining of human kidney using 12542-1-AP

IHC staining of human kidney using 12542-1-AP

IF Staining of HepG2 using 12542-1-AP

Annexin VI Polyclonal antibody

Annexin VI Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Isolation and Comprehensive Analysis of Cochlear Tissue-Derived Small Extracellular Vesicles

Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion-exchange method.

CD300A promotes tumor progression by PECAM1, ADCY7 and AKT pathway in acute myeloid leukemia.

Dynamic Mitochondrial Proteome Under Polyamines Treatment in Cardiac Aging.

TMT-based proteomics analysis of the cerebral cortex of TauT knockout rats.

A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis.