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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon tissue using ANXA5 Recombinant antibody (83929-2-RR)

    IHC staining of human colon using 83929-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 83929-2-RR (ANXA5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using ANXA5 Recombinant antibody (83929-2-RR)

    IHC staining of human colon using 83929-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 83929-2-RR (ANXA5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ANXA5 Recombinant antibody (83929-2-RR)

    IF Staining of HeLa using 83929-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ANXA5 antibody (83929-2-RR, Clone: 240621B10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of PC-3 cells using ANXA5 Recombinant antibody (83929-2-RR)

    FC experiment of PC-3 using 83929-2-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug Anxa5 Recombinant Antibody (83929-2-RR, Clone:240621B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Flow cytometry (FC) experiment of HeLa cells using ANXA5 Recombinant antibody (83929-2-RR)

    FC experiment of HeLa using 83929-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anxa5 Recombinant Antibody (83929-2-RR, Clone:240621B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83929-2-RR

    Affinity and Kinetic Characterization of 83929-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83929-2-RR against Human ANXA5 were performed. The affinity constant is 18.2 nM.

Annexin V Recombinant monoclonal antibody

Annexin V Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83929-2-RR
Clone No.240621B10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Annexin A5, ANXA5, 240621B10, Anchorin CII, Annexin 5

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inPC-3 cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83929-2-RR targets Annexin V in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0741

Product name: Recombinant human 0741 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-320 aa of BC001429

Sequence: MAQVLRGTVTDFPGFDERADAETLRKAMKGLGTDEESILTLLTSRSNAQRQEISAAFKTLFGRDLLDDLKSELTGKFEKLIVALMKPSRLYDAYELKHALKGAGTNEKVLTEIIASRTPEELRAIKQVYEEEYGSSLEDDVVGDTSGYYQRMLVVLLQANRDPDAGIDEAQVEQDAQALFQAGELKWGTDEEKFITIFGTRSVSHLRKVFDKYMTISGFQIEETIDRETSGNLEQLLLAVVKSIRSIPAYLAETLYYAMKGAGTDDHTLIRVMVSRSEIDLFNIRKEFRKNFATSLYSMIKGDTSGDYKKALLLLCGEDD

相同性解析による交差性が予測される生物種
Full Name annexin A5
Calculated molecular weight 36 kDa
Observed molecular weight 32-36 kDa
GenBank accession numberBC001429
Gene Symbol Annexin V
Gene ID (NCBI) 308
RRIDAB_3671510
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP08758
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.

Protocols

Product Specific Protocols
IF protocol for Annexin V antibody 83929-2-RRDownload protocol
IHC protocol for Annexin V antibody 83929-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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