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Annexin A11 Polyclonal antibody
Annexin A11 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10479-2-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, mouse uterus tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human ovary tumor tissue, human breast cancer tissue, human kidney tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 4 publications below |
IHC | See 6 publications below |
IF | See 3 publications below |
Product Information
10479-2-AP targets Annexin A11 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Annexin A11 fusion protein Ag0729 相同性解析による交差性が予測される生物種 |
Full Name | annexin A11 |
Calculated molecular weight | 56 kDa |
Observed molecular weight | 56-60 kDa |
GenBank accession number | BC007564 |
Gene symbol | ANXA11 |
Gene ID (NCBI) | 311 |
RRID | AB_2057171 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Annexin A11 (Anxa11) is one member of annexins that are Ca2+-regulated phospholipid-binding proteins. Annexin A11 plays an important role in cell division, differentiation, apoptosis, vesicle trafficking and Ca2+ signaling. Annexin A11 dysregulation is involved in the progression, drug-resistance, recurrence of systemic autoimmune disease and cancer.
Protocols
Product Specific Protocols | |
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WB protocol for Annexin A11 antibody 10479-2-AP | Download protocol |
IHC protocol for Annexin A11 antibody 10479-2-AP | Download protocol |
IF protocol for Annexin A11 antibody 10479-2-AP | Download protocol |
IP protocol for Annexin A11 antibody 10479-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Sci Transl Med Mutations in the vesicular trafficking protein annexin A11 are associated with amyotrophic lateral sclerosis. | ||
Oncotarget ANXA11 regulates the tumorigenesis, lymph node metastasis and 5-fluorouracil sensitivity of murine hepatocarcinoma Hca-P cells by targeting c-Jun.
| ||
Biomed Pharmacother Annexin A11 knockdown inhibits in vitro proliferation and enhances survival of Hca-F cell via Akt2/FoxO1 pathway and MMP-9 expression.
| ||
Neurobiol Aging Genetic screening of ANXA11 revealed novel mutations linked to amyotrophic lateral sclerosis. | ||
Ann Clin Transl Neurol Adult-onset dominant muscular dystrophy in Greek families caused by Annexin A11 |