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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANUBL1 Polyclonal antibody (17478-1-AP)

    WB analysis using 17478-1-AP (same clone as 17478-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17478-1-AP (ANUBL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17478-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ANUBL1 Polyclonal antibody (17478-1-AP)

    WB analysis using 17478-1-AP (same clone as 17478-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17478-1-AP (ANUBL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17478-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ANUBL1 Polyclonal antibody (17478-1-AP)

    IHC staining of human stomach cancer using 17478-1-AP (same clone as 17478-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17478-1-AP (ANUBL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17478-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ANUBL1 Polyclonal antibody (17478-1-AP)

    IHC staining of human stomach cancer using 17478-1-AP (same clone as 17478-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17478-1-AP (ANUBL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17478-1-PBS in a different storage buffer formulation.

ANUBL1 Polyclonal antibody, PBS Only

ANUBL1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 17478-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17478-1-PBS targets ANUBL1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11505

Product name: Recombinant human ANUBL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 305-653 aa of BC048968

Sequence: GNIVLPSEECVTEQSLLPKVGSLASFAEGNADEQSSGLEGACKVNLELLLTNADKGLKAPEQHLKHVAGVLNGESVETSVLNYRELSPHKNRLLSPLRCSAPMSLHNSLVKPERQSKCFEFGKLQPSSSQSLDVQNITDSSFSRTTCFQGVKVDSLGKRSDVISKVEARDITEMTNKASKEPVGCVNNISFLASLAGSTSRNRLQSTRGAGRLQNSGTGLSTNLQHFQEENFRKSSPQLEHTGVFLSTHGVGMNGNNAAAGKSVGECTTHHLPPVKAPLQTKKKTTNHCFLCGKKTGLASSYECRCGNNFCASHRYAETHGCTYDYKSAGRRYLHEANPVVNAPKLPKI

相同性解析による交差性が予測される生物種
Full Name AN1, ubiquitin-like, homolog (Xenopus laevis)
Calculated molecular weight 653 aa, 72 kDa
Observed molecular weight70-80 kDa
GenBank accession numberBC048968
Gene Symbol ANUBL1
Gene ID (NCBI) 93550
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86XD8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ANUBL1 is also named as ZFAND4. The expression of ANUBL1 was specifically upregulated in gastric cancer and it was positively associated with grades of gastric cancer. Moreover, it promoted proliferation of gastric cancer SGC-7901 cells. The mechanism of ANUBL1 upregulation in gastric cancer is that miR-182 suppressed the expression of ANUBL1 through targeting its 3'UTR, and ANUBL1 could downregulate the expression of anti-proliferation-associated miRNAs (miR-148b, miR-375 and miR-182) in SGC-7901 cells. The calculated molecular weight of ANUBL1 is 80 kDa (PMID: 25682742).

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