Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of HEK-293 using 16723-1-AP (same clone as 16723-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16723-1-AP (ANTXR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16723-1-PBS in a different storage buffer formulation.
WB analysis of PC-3 using 16723-1-AP (same clone as 16723-1-PBS)
PC-3 cells were subjected to SDS PAGE followed by western blot with 16723-1-AP (ANTXR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16723-1-PBS in a different storage buffer formulation.
IP experiment of HEK-293 using 16723-1-AP (same clone as 16723-1-PBS)
IP result of anti-ANTXR2 (IP:16723-1-AP, 4ug; Detection:16723-1-AP 1:500) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 16723-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 16723-1-AP (same clone as 16723-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 16723-1-AP (ANTXR2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 16723-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 16723-1-AP (same clone as 16723-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 16723-1-AP (ANTXR2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 16723-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16723-1-AP (same clone as 16723-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 16723-1-AP (ANTXR2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16723-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16723-1-AP (same clone as 16723-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 16723-1-AP (ANTXR2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16723-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 16723-1-AP (same clone as 16723-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 16723-1-AP (ANTXR2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16723-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 16723-1-AP (same clone as 16723-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 16723-1-AP (ANTXR2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16723-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 16723-1-AP (same clone as 16723-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 16723-1-AP (ANTXR2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16723-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 16723-1-AP (same clone as 16723-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 16723-1-AP (ANTXR2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16723-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 16723-1-AP (same clone as 16723-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 16723-1-AP (ANTXR2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16723-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 16723-1-AP (same clone as 16723-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 16723-1-AP (ANTXR2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16723-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

ANTXR2 Polyclonal antibody, PBS Only

ANTXR2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 16723-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16723-1-PBS targets ANTXR2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10162 Product name: Recombinant human ANTXR2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 25-231 aa of BC107876 Sequence: ISKGLEDLKRVSPVGETYIHEGLKLANEQIQKAGGLKTSSIIIALTDGKLDGLVPSYAEKEAKISRSLGASVYCVGVLDFEQAQLERIADSKEQVFPVKGGFQALKGIINSILAQSCTEILELQPSSVCVGEEFQIVLSGRGFMLGSRNGSVLCTYTVNETYTTSVKPVSVQLNSMLCPAPILNKAGETLDVSVSFNGGKSVISGSL 相同性解析による交差性が予測される生物種
Full Name	anthrax toxin receptor 2
Calculated molecular weight	54 kDa
Observed molecular weight	55 kDa
GenBank accession number	BC107876
Gene Symbol	ANTXR2
Gene ID (NCBI)	118429
RRID	AB_2056741
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P58335
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

ANTXR2 (anthrax toxin receptor 2), also known as CMG2 (capillary morphogenesis gene 2 protein) is a transmembrane protein that is induced during capillary morphogenesis. It contains a vWA domain that shows strong binding to laminin and collagen IV, suggesting that this protein plays a role in basement membrane matrix assembly and endothelial cell morphogenesis (PMID: 11683410; 14508707). Like its paralog TEM8 (ANTXR1), ANTXR2 also functions as a receptor for anthrax toxin. Mutations in ANTXR2 result in the allelic disorders juvenile hyaline fibromatosis and infantile systemic hyalinosis (PMID: 14508707; 12973667; 22383261).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 16723-1-AP (same clone as 16723-1-PBS)

WB analysis of PC-3 using 16723-1-AP (same clone as 16723-1-PBS)

IP experiment of HEK-293 using 16723-1-AP (same clone as 16723-1-PBS)

IHC staining of human ovary using 16723-1-AP (same clone as 16723-1-PBS)

IHC staining of human ovary using 16723-1-AP (same clone as 16723-1-PBS)

IHC staining of human kidney using 16723-1-AP (same clone as 16723-1-PBS)

IHC staining of human kidney using 16723-1-AP (same clone as 16723-1-PBS)

IHC staining of human placenta using 16723-1-AP (same clone as 16723-1-PBS)