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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using ANT1/2 Polyclonal antibody (15997-1-AP)

    WB analysis of human heart using 15997-1-AP (same clone as 15997-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 15997-1-AP (ANT1/2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15997-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using ANT1/2 Polyclonal antibody (15997-1-AP)

    WB analysis of MCF-7 using 15997-1-AP (same clone as 15997-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 15997-1-AP (ANT1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15997-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using ANT1/2 Polyclonal antibody (15997-1-AP)

    WB analysis of mouse skeletal muscle using 15997-1-AP (same clone as 15997-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15997-1-AP (ANT1/2antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15997-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ANT1/2 Polyclonal antibody (15997-1-AP)

    WB analysis of mouse brain using 15997-1-AP (same clone as 15997-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15997-1-AP (ANT1/2antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15997-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using ANT1/2 Polyclonal antibody (15997-1-AP)

    IP experiment of mouse heart using 15997-1-AP (same clone as 15997-1-PBS)

    IP result of anti-ANT1/2 (IP:15997-1-AP, 4ug; Detection:15997-1-AP 1:500) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 15997-1-PBS in a different storage buffer formulation.

ANT1/2 Polyclonal antibody, PBS Only

ANT1/2 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 15997-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

Synonyms

SLC25A4, AAC1, ADP,ATP carrier protein 1, ADP/ATP translocase 1, ANT

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15997-1-PBS targets ANT1/2 in applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7787

Product name: Recombinant human SLC25A4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-298 aa of BC008664

Sequence: MGDHAWSFLKDFLAGGVAAAVSKTAVAPIERVKLLLQVQHASKQISAEKQYKGIIDCVVRIPKEQGFLSFWRGNLANVIRYFPTQALNFAFKDKYKQLFLGGVDRHKQFWRYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKGAAQREFHGLGDCIIKIFKSDGLRGLYQGFNVSVQGIIIYRAAYFGVYDTAKGMLPDPKNVHIFVSWMIAQSVTAVAGLVSYPFDTVRRRMMMQSGRKGADIMYTGTVDCWRKIAKDEGAKAFFKGAWSNVLRGMGGAFVLVLYDEIKKYV

相同性解析による交差性が予測される生物種
Full Name solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 4
Calculated molecular weight 33 kDa
Observed molecular weight33 kDa
GenBank accession numberBC008664
Gene Symbol SLC25A4
Gene ID (NCBI) 291
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP12235
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2.

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