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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANP32E Polyclonal antibody (31989-1-AP)

    WB analysis using 31989-1-AP (same clone as 31989-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31989-1-AP (ANP32E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31989-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using ANP32E Polyclonal antibody (31989-1-AP)

    IHC staining of human lung using 31989-1-AP (same clone as 31989-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 31989-1-AP (ANP32E antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31989-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using ANP32E Polyclonal antibody (31989-1-AP)

    IHC staining of mouse small intestine using 31989-1-AP (same clone as 31989-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 31989-1-AP (ANP32E antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31989-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ANP32E Polyclonal antibody (31989-1-AP)

    IHC staining of mouse kidney using 31989-1-AP (same clone as 31989-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31989-1-AP (ANP32E antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31989-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using ANP32E Polyclonal antibody (31989-1-AP)

    IF Staining of mouse small intestine using 31989-1-AP (same clone as 31989-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using ANP32E antibody (31989-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31989-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using ANP32E Polyclonal antibody (31989-1-AP)

    IF Staining of mouse small intestine using 31989-1-AP (same clone as 31989-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using ANP32E antibody (31989-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31989-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using ANP32E Polyclonal antibody (31989-1-AP)

    IF Staining of SH-SY5Y using 31989-1-AP (same clone as 31989-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using ANP32E antibody (31989-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 31989-1-PBS in a different storage buffer formulation.

ANP32E Polyclonal antibody, PBS Only

ANP32E Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 31989-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

Acidic (Leucine-Rich) Nuclear Phosphoprotein 32 Family, Member E, Acidic leucine-rich nuclear phosphoprotein 32 family member E, Acidic Nuclear Phosphoprotein 32 Family Member E, LANPL, LANP-L

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31989-1-PBS targets ANP32E in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag33772

Product name: Recombinant human ANP32E protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 159-268 aa of BC003380

Sequence: EEEDDEDGDEDDEEEEENEAGPPEGYEEEEEEEEEEDEDEDEDEDEAGSELGEGEEEVGLSYLMKEEIQDEEDDDDYVEEGEEEEEEEEGGLRGEKRKRDAEDDGEEEDD*

相同性解析による交差性が予測される生物種
Full Name acidic (leucine-rich) nuclear phosphoprotein 32 family, member E
Calculated molecular weight 31 kDa
Observed molecular weight30 kDa
GenBank accession numberBC003380
Gene Symbol ANP32E
Gene ID (NCBI) 81611
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9BTT0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Acidic nuclear phosphoprotein 32 family member E (ANP32E) is a histone chaperone that removes H2A.Z from chromatin. ANP32E is implicated in many cellular processes, including chromatin modification and remodeling, gene regulation, protein phosphorylation, and regulation of intracellular trafficking, and contributes to early development and cancer metastasis in mammals. In addition, ANP32E is involved in tumor development and is highly expressed in pancreatic and thyroid cancer cells, and promotes tumorigenesis in gastric cancer (GC) cells by inducing NUF2 expression.

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