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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANP32CP Recombinant antibody (84553-1-RR)

    WB analysis using 84553-1-RR (same clone as 84553-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84553-1-RR (ANP32CP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84553-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using ANP32CP Recombinant antibody (84553-1-RR)

    IF Staining of LNCaP using 84553-1-RR (same clone as 84553-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using ANP32CP antibody (84553-1-RR, Clone: 241562D12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84553-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84553-1-RR

    Affinity and Kinetic Characterization of 84553-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84553-1-RR against Human ANP32CP were performed. The affinity constant is 7.76 nM.

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ANP32CP Recombinant antibody, PBS Only

ANP32CP Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84553-1-PBS
Clone No.241562D12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ANP32C, Tumorigenic protein pp32r1, PP32R1, 241562D12

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84553-1-PBS targets ANP32CP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ANP32CP fusion protein Ag35293 相同性解析による交差性が予測される生物種
Full Name acidic (leucine-rich) nuclear phosphoprotein 32 family, member C
Calculated molecular weight27 kDa
Observed molecular weight27-30 kDa
GenBank accession numberNM_012403.1
Gene Symbol ANP32C/PP32R1
Gene ID (NCBI) 23520
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO43423
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ANP32CP (acidic nuclear phosphoprotein 32 family member C, pseudogene), also known as PP32R1. It is expected to be located in nucleus and perinuclear region of cytoplasm. Expressed in activated stem cells, such as mobilized CD34+ cells and cord blood CD34+ cells, but not in resting bone marrow CD34+ cells. Expressed in a variety of neoplastic cell lines, mainly in prostatic adenocarcinoma cell lines. Not expressed in normal prostatic tissue (PMID: 10086381). The calculated molecular weight of ANP32CP is 27 kDa. The protein could be the product of a pseudogene. Gene encoding ANP32CP is intronless suggesting that is pseudogene generated by retrotransposition.