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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANP32CP Polyclonal antibody (31298-1-AP)

    WB analysis using 31298-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31298-1-AP (ANP32CP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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ANP32CP Polyclonal antibody

ANP32CP Polyclonal Antibody for WB, ELISA
Cat No. 31298-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

ANP32C, PP32R1, Tumorigenic protein pp32r1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inDU 145 cells, LNCaP cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31298-1-AP targets ANP32CP in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANP32CP fusion protein Ag35293 相同性解析による交差性が予測される生物種
Full Name acidic (leucine-rich) nuclear phosphoprotein 32 family, member C
Calculated molecular weight27 kDa
Observed molecular weight27-30 kDa
GenBank accession numberNM_012403.1
Gene Symbol ANP32C/PP32R1
Gene ID (NCBI) 23520
RRIDAB_3669935
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO43423
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANP32CP (acidic nuclear phosphoprotein 32 family member C, pseudogene), also known as PP32R1. It is expected to be located in nucleus and perinuclear region of cytoplasm. Expressed in activated stem cells, such as mobilized CD34+ cells and cord blood CD34+ cells, but not in resting bone marrow CD34+ cells. Expressed in a variety of neoplastic cell lines, mainly in prostatic adenocarcinoma cell lines. Not expressed in normal prostatic tissue (PMID: 10086381). The calculated molecular weight of ANP32CP is 27 kDa. The protein could be the product of a pseudogene. Gene encoding ANP32CP is intronless suggesting that is pseudogene generated by retrotransposition.

Protocols

Product Specific Protocols
WB protocol for ANP32CP antibody 31298-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols