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Validation Data Gallery

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  • Sandwich ELISA standard curve of MP50363-3

    Sandwich ELISA standard curve of MP50363-3

    Sandwich ELISA standard curve of MP50363-3, Human ANP32B Monoclonal Matched Antibody Pair - PBS only. 66160-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1274. 66160-2-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

  • Sample test of MP50363-3

    Sample test of MP50363-3

    The mean ANP32B concentration was determined to be 105.34 ng/mL in HCT116 cell extract based on a 2.0 mg/mL extract load, 83.16 ng/mL in A431 cell extract based on a 1.3 mg/mL extract load and 84.78 ng/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

  • Cytometric bead array standard curve of MP50363-2

    Cytometric bead array standard curve of MP50363-2

    Cytometric bead array standard curve of MP50363-2, ANP32B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66160-1-PBS. Detection antibody: 66160-3-PBS. Standard:Ag1274. Range: 1.563-200 ng/mL.

  • Cytometric bead array standard curve of MP50363-3

    Cytometric bead array standard curve of MP50363-3

    Cytometric bead array standard curve of MP50363-3, ANP32B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66160-3-PBS. Detection antibody: 66160-2-PBS. Standard:Ag1274. Range: 1.563-200 ng/mL.

ANP32B Monoclonal antibody, PBS Only (Capture/Detector)

ANP32B Monoclonal Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 66160-3-PBS
Clone No.1H3E5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Silver-stainable protein SSP29, Putative HLA-DR-associated protein I-2, PHAPI2, APRIL, Acidic leucine-rich nuclear phosphoprotein 32 family member B

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66160-3-PBS targets ANP32B as part of a matched antibody pair:

MP50363-2: 66160-1-PBS capture and 66160-3-PBS detection (validated in Cytometric bead array)

MP50363-3: 66160-3-PBS capture and 66160-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag1274

Product name: Recombinant human ANP32B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-177 aa of BC013003

Sequence: MDMKRRIHLELRNRTPAAVRELVLDNCKSNDGKIEGLTAEFVNLEFLSLINVGLISVSNLPKLPKLKKLELSENRIFGGLDMLAEKLPNLTHLNLSGNKLKDISTLEPLKKLECLKSLDLFNCEVTNLNDYRESVFKLLPQLTYLDGYDREDQEAPDSDAEVDGVDEEEEDEEGEDEEDEDDEDGEEEEFDEEDDEDEDVEGDEDDDEVSEEEEEFGLDEEDEDEDEDEEEEEGGKGEKRKRETDDEGEDD

相同性解析による交差性が予測される生物種
Full Name acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
Calculated molecular weight 29 kDa
GenBank accession numberBC013003
Gene Symbol ANP32B
Gene ID (NCBI) 10541
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ92688
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.