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  • Featured Product
  • KD/KO Validated

ANP32A Polyclonal antibody

ANP32A Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15810-1-AP

Synonyms

ANP32A, C15orf1, I1PP2A, LANP, MAPM, Mapmodulin, PHAP1, PHAPI, PP32

Validation Data Gallery

Applications:
  • WB analysis using 15810-1-AP

    WB analysis using 15810-1-AP

    Various cells were subjected to SDS PAGE followed by western blot with 15810-1-AP (ANP32A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 15810-1-AP

    WB analysis using 15810-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15810-1-AP (ANP32A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 15810-1-AP

    WB analysis of human brain using 15810-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15810-1-AP (ANP32A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse thymus using 15810-1-AP

    WB analysis of mouse thymus using 15810-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 15810-1-AP (ANP32A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 15810-1-AP

    WB analysis using 15810-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15810-1-AP (ANP32A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis using 15810-1-AP

    WB analysis using 15810-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15810-1-AP (ANP32A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 15810-1-AP

    IHC staining of human kidney using 15810-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15810-1-AP (ANP32A Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with ANP32A antibody included
  • IHC staining of human kidney using 15810-1-AP

    IHC staining of human kidney using 15810-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15810-1-AP (ANP32A Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with ANP32A antibody included
  • IF Staining of A431 using 15810-1-AP

    IF Staining of A431 using 15810-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 15810-1-AP (ANP32A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of A2780 using 15810-1-AP

    IF Staining of A2780 using 15810-1-AP

    Immunofluorescent analysis of A2780 cells, using ANP32A antibody 15810-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inA549 cells, human brain tissue, mouse brain tissue, mouse thymus tissue, Raji cells, HeLa cells, MCF-7 cells, HEK-293T cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells, A2780 cells
Planning an IHC experiment? We recommend our IHCeasy ANP32A Ready-To-Use IHC Kit. ANP32A primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15810-1-AP targets ANP32A in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANP32A fusion protein Ag8546 相同性解析による交差性が予測される生物種
Full Name acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
Calculated molecular weight 249 aa, 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC007200
Gene symbol ANP32A
Gene ID (NCBI) 8125
RRIDAB_2056302
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANP32A, an acidic leucine-rich nuclear phosphoprotein, is a member of the inhibitor of the histone acetyltransferase (INHAT) complex and preferentially binds to unmodified histone H3 tails in vitro. ANP32A is expressed in normal tissues as well as in pancreatic, breast and prostate cancer and other malignant tumors. ANP32A plays an important role in cell proliferation, apoptosis, transcriptional regulation, signal transduction and other processes.

Protocols

Product Specific Protocols
WB protocol for ANP32A antibody 15810-1-APDownload protocol
IHC protocol for ANP32A antibody 15810-1-APDownload protocol
IF protocol for ANP32A antibody 15810-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Arch Virol

The interaction of cellular protein ANP32A with influenza A virus polymerase component PB2 promotes vRNA synthesis.

Authors - Xiuli Wei
  • KD Validated
View Article