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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANO2 Monoclonal antibody (67638-1-Ig)

    WB analysis using 67638-1-Ig (same clone as 67638-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67638-1-Ig (ANO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67638-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCCIT cells using ANO2 Monoclonal antibody (67638-1-Ig)

    WB analysis of NCCIT using 67638-1-Ig (same clone as 67638-1-PBS)

    NCCIT cells were subjected to SDS PAGE followed by western blot with 67638-1-Ig (ANO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67638-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using ANO2 Monoclonal antibody (67638-1-Ig)

    IHC staining of mouse eye using 67638-1-Ig (same clone as 67638-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67638-1-Ig (ANO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67638-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using ANO2 Monoclonal antibody (67638-1-Ig)

    IHC staining of mouse eye using 67638-1-Ig (same clone as 67638-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67638-1-Ig (ANO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67638-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using ANO2 Monoclonal antibody (67638-1-Ig)

    IF Staining of mouse eye using 67638-1-Ig (same clone as 67638-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using ANO2 antibody (67638-1-Ig, Clone: 2F12G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67638-1-PBS in a different storage buffer formulation.

ANO2 Monoclonal antibody, PBS Only

ANO2 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67638-1-PBS
Clone No.2F12G1

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

ANO2, anoctamin 2, C12orf3, DKFZp434P102, TMEM16B, Transmembrane protein 16B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67638-1-PBS targets ANO2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name anoctamin 2
Calculated molecular weight 114 kDa
Observed molecular weight 114 kDa
GenBank accession numberNM_020373
Gene Symbol ANO2
Gene ID (NCBI) 57101
RRIDAB_2882839
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9NQ90
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ANO2, also named as C12orf3 and TMEM16B, belongs to the anoctamin family. It acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. It may mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina.

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