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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANO2 Polyclonal antibody (20647-1-AP)

    WB analysis using 20647-1-AP (same clone as 20647-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 20647-1-AP (ANO2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using ANO2 Polyclonal antibody (20647-1-AP)

    IP experiment of mouse brain using 20647-1-AP (same clone as 20647-1-PBS)

    IP result of anti-ANO2 (IP:20647-1-AP, 4ug; Detection:20647-1-AP 1:300) with mouse brain tissue lysate 10000ug. This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using ANO2 Polyclonal antibody (20647-1-AP)

    IHC staining of mouse eye using 20647-1-AP (same clone as 20647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 20647-1-AP (ANO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using ANO2 Polyclonal antibody (20647-1-AP)

    IHC staining of mouse eye using 20647-1-AP (same clone as 20647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 20647-1-AP (ANO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat eye tissue using ANO2 Polyclonal antibody (20647-1-AP)

    IHC staining of rat eye using 20647-1-AP (same clone as 20647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 20647-1-AP (ANO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using ANO2 Polyclonal antibody (20647-1-AP)

    IHC staining of mouse eye using 20647-1-AP (same clone as 20647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 20647-1-AP (ANO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ANO2 Polyclonal antibody (20647-1-AP)

    IHC staining of human breast cancer using 20647-1-AP (same clone as 20647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20647-1-AP (ANO2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ANO2 Polyclonal antibody (20647-1-AP)

    IHC staining of human breast cancer using 20647-1-AP (same clone as 20647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20647-1-AP (ANO2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat eye tissue using ANO2 Polyclonal antibody (20647-1-AP)

    IF Staining of rat eye using 20647-1-AP (same clone as 20647-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using ANO2 antibody (20647-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ANO2 Polyclonal antibody, PBS Only

ANO2 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 20647-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

anoctamin 2, Anoctamin-2, C12orf3, TMEM16B, Transmembrane protein 16B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20647-1-PBS targets ANO2 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name anoctamin 2
Calculated molecular weight 114 kDa
Observed molecular weight 114 kDa
GenBank accession numberNM_020373
Gene Symbol ANO2
Gene ID (NCBI) 57101
RRIDAB_10696181
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQ90
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ANO2, also named as C12orf3 and TMEM16B, belongs to the anoctamin family. It acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. It may mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina.

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