Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, fetal human brain tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | mouse ovary tissue, human kidney tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 2 publications below |
Product Information
24333-1-AP targets ANKRD6 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19328 Product name: Recombinant human ANKRD6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-260 aa of BC042173 Sequence: MSQQDAVAALSERLLVAAYKGQTENVVQLINKGARVAVTKHGRTPLHLAANKGHLPVVQILLKAGCDLDVQDDGDQTALHRATVVGNTEIIAALIHEGCALDRQDKDGNTALHEASWHGFSQSAKLLVKAGANVLAKNKAGNTALHLACQNSHSQSTRVLLLAGSRADLKNNAGDTCLHVAARYNHLSIIRLLLTAFCSVHEKNQAGDTALHVAAALNHKKVAKILLEAGADTTIVNNAGQTPLETARYHNNPEVALLLT 相同性解析による交差性が予測される生物種 |
| Full Name | ankyrin repeat domain 6 |
| Calculated molecular weight | 727 aa, 80 kDa |
| Observed molecular weight | 80-90 kDa |
| GenBank accession number | BC042173 |
| Gene Symbol | ANKRD6 |
| Gene ID (NCBI) | 22881 |
| RRID | AB_2879498 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2G4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ankrd6 (Ankyrin repeat domain 6) is a distant homolog of the fly PCP mediator diego and acts as a molecular switch between both Wnt pathways. It promotes signaling of the non canonical Wnt/PCP pathway and simultaneously inhibits gene transcription through the canonical Wnt/β-catenin pathway.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ANKRD6 antibody 24333-1-AP | Download protocol |
| IP protocol for ANKRD6 antibody 24333-1-AP | Download protocol |
| WB protocol for ANKRD6 antibody 24333-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Chin J Cancer Res Pan-cancer analyses demonstrate that ANKRD6 is associated with a poor prognosis and correlates with M2 macrophage infiltration in colon cancer. | ||
Mol Carcinog Role of miR-214 in regulation of β-catenin and the malignant phenotype of melanoma.
| ||
Reprod Biol Endocrinol Map-1a regulates Sertoli cell BTB dynamics through the cytoskeletal organization of microtubule and F-actin | ||
Biomedicines The Roles of AGTRAP, ALKBH3, DIVERSIN, NEDD8 and RRM1 in Glioblastoma Pathophysiology and Prognosis |
