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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANKRD55 Polyclonal antibody (24203-1-AP)

    WB analysis using 24203-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24203-1-AP (ANKRD55 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ANKRD55 Polyclonal antibody (24203-1-AP)

    WB analysis of HeLa using 24203-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24203-1-AP (ANKRD55 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using ANKRD55 Polyclonal antibody (24203-1-AP)

    WB analysis of L02 using 24203-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 24203-1-AP (ANKRD55 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ANKRD55 Polyclonal antibody (24203-1-AP)

    IHC staining of human cervical cancer using 24203-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24203-1-AP (ANKRD55 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ANKRD55 Polyclonal antibody (24203-1-AP)

    IHC staining of human cervical cancer using 24203-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24203-1-AP (ANKRD55 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ANKRD55 Polyclonal antibody (24203-1-AP)

    IF Staining of HeLa using 24203-1-AP

    Immunofluorescent analysis of HeLa cells using 24203-1-AP (ANKRD55 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ANKRD55 Polyclonal antibody

ANKRD55 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24203-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Ankyrin repeat domain-containing protein 55

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, L02 cells, mouse spleen tissue, mouse small intestine tissue, mouse thymus tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24203-1-AP targets ANKRD55 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21271

Product name: Recombinant human ANKRD55 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-326 aa of BC106914

Sequence: MDSNLRDINESTPLAYALYCGHTACVKLLSQESRTEPTRPPPSQSSRPQKKERRFNVLNQIFCKNKKEEQRAHQKDPSRDRYREEDTSEVNDIITTFDSIVGTNCQEQPGDQVAMVEFKKKTSDNSKYLLPEKKPLARKGLPPIRTQSLPPITLGNNFLTASHRATSHAGLSSAPHHMAQRSQKSRSEQDLLNNRTGCQMLLDNPWKSDSNQVFSYKVWTVSSSDKLLDRLLSVRPGHQEVSVPPHLRHLHNPSSGQNFQHLSPNRHKIRDLPFTRNNLAPLPDQKFLSGEPLRTNRVLPAIPSQRRHSTAAEESEHSANPTSDEN

相同性解析による交差性が予測される生物種
Full Name ankyrin repeat domain 55
Calculated molecular weight 614 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC106914
Gene Symbol ANKRD55
Gene ID (NCBI) 79722
RRIDAB_2879456
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3KP44
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANKRD55 also named as Ankyrin repeat domain containing protein 55 is a 614 amino acid protein, which contains 9 ANK repeat. ANKRD55 may exist three alternatively spliced isoforms and belongs to ankyrin family. The function of ANKRD55 is still non-known and need to be further studied. ANKRD55 is a critical regulator of TCR signaling and a key regulator of T cell inflammation in multiple sclerosis (PMID: 41090353).

Protocols

Product Specific Protocols
IF protocol for ANKRD55 antibody 24203-1-APDownload protocol
IHC protocol for ANKRD55 antibody 24203-1-APDownload protocol
WB protocol for ANKRD55 antibody 24203-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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