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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANKRD54 Monoclonal antibody (68388-1-Ig)

    WB analysis using 68388-1-Ig (same clone as 68388-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68388-1-Ig (ANKRD54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68388-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using ANKRD54 Monoclonal antibody (68388-1-Ig)

    WB analysis of U2OS using 68388-1-Ig (same clone as 68388-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 68388-1-Ig (ANKRD54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68388-1-PBS in a different storage buffer formulation.

ANKRD54 Monoclonal antibody, PBS Only

ANKRD54 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68388-1-PBS
Clone No.1E9A9

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, Indirect ELISA

Synonyms

ANKRD54, ankyrin repeat domain 54

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68388-1-PBS targets ANKRD54 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag22772

Product name: Recombinant human ANKRD54 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 201-300 aa of BC066909

Sequence: RVDALDRAGRTPLHLAKSKLNILQEGHAQCLEAVRLEVKQIIHMLREYLERLGQHEQRERLDDLCTRLQMTSTKEQVDEVTDLLASFTSLSLQMQSMEKR

相同性解析による交差性が予測される生物種
Full Name ankyrin repeat domain 54
Calculated molecular weight 300 aa, 33 kDa
Observed molecular weight 33-35 kDa
GenBank accession numberBC066909
Gene Symbol ANKRD54
Gene ID (NCBI) 129138
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ6NXT1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Ankyrin repeat domain 54 (ANKRD54)/Liar is a nuclear-cytoplasmic shuttling adaptor that interacts with Lyn, Btk, Txk, and HCLS1. Activation of PKC kinases promoted nuclear export and phosphorylation of Ankrd54, and increased interaction and cytoplasmic co-localization with Lyn.

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