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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ANKRD53 Polyclonal antibody (24283-1-AP)

    WB analysis of HeLa using 24283-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24283-1-AP (ANKRD53 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ANKRD53 Polyclonal antibody (24283-1-AP)

    WB analysis of HepG2 using 24283-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24283-1-AP (ANKRD53 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ANKRD53 Polyclonal antibody (24283-1-AP)

    IHC staining of human kidney using 24283-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24283-1-AP (ANKRD53 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ANKRD53 Polyclonal antibody (24283-1-AP)

    IHC staining of human kidney using 24283-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24283-1-AP (ANKRD53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ANKRD53 Polyclonal antibody (24283-1-AP)

    IHC staining of mouse testis using 24283-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24283-1-AP (ANKRD53 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ANKRD53 Polyclonal antibody (24283-1-AP)

    IHC staining of mouse testis using 24283-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24283-1-AP (ANKRD53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ANKRD53 Polyclonal antibody (24283-1-AP)

    IF Staining of HepG2 using 24283-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 24283-1-AP (ANKRD53 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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ANKRD53 Polyclonal antibody

ANKRD53 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24283-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Ankyrin repeat domain-containing protein 53, ankyrin repeat domain 53

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24283-1-AP targets ANKRD53 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKRD53 fusion protein Ag15030 相同性解析による交差性が予測される生物種
Full Name ankyrin repeat domain 53
Calculated molecular weight 530 aa, 60 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC035234
Gene Symbol ANKRD53
Gene ID (NCBI) 79998
RRIDAB_2879470
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N9V6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANKRD53 also termed as ankyrin repeat domain 53 is a 533 amino-acid protein,which contains three ANK repeats and belongs to ankyrin family. The function of ANKRD53 is still non-known and need to be further studied. Ankyrins are membrane adaptor molecules that play important roles in coupling integral membrane proteins to the spectrin-based cytoskeleton network.

Protocols

Product Specific Protocols
WB protocol for ANKRD53 antibody 24283-1-APDownload protocol
IHC protocol for ANKRD53 antibody 24283-1-APDownload protocol
IF protocol for ANKRD53 antibody 24283-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols