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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANKRD46 Recombinant monoclonal antibody (86827-4-RR)

    WB analysis using 86827-4-RR (same clone as 86827-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86827-4-RR (ANKRD46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86827-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ANKRD46 Recombinant monoclonal antibody (86827-4-RR)

    WB analysis using 86827-4-RR (same clone as 86827-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86827-4-RR (ANKRD46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86827-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ANKRD46 Recombinant monoclonal antibody (86827-4-RR)

    IF Staining of HEK-293 using 86827-4-RR (same clone as 86827-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ANKRD46 antibody (86827-4-RR, Clone: 251840D6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86827-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using ANKRD46 Recombinant monoclonal antibody (86827-4-RR)

    IF Staining of Jurkat using 86827-4-RR (same clone as 86827-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ANKRD46 antibody (86827-4-RR, Clone: 251840D6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86827-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86827-4-RR

    Affinity and Kinetic Characterization of 86827-4-RR

    Biolayer interferometry (BLl) kinetic assays of 86827-4-RR against Human ANKRD46 were performed. The affinity constant is 0.351 nM.

ANKRD46 Recombinant monoclonal antibody, PBS Only

ANKRD46 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86827-4-PBS
Clone No.251840D6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ANK-S, ankyrin repeat domain 46, Ankyrin repeat domain-containing protein 46

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86827-4-PBS targets ANKRD46 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag26623

Product name: Recombinant human ANKRD46 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-189 aa of BC060843

Sequence: MSYVFVNDSSQTNVPLLQACIDGDFNYSKRLLESGFDPNIRDSRGRTGLHLAAARGNVDICQLLHKFGADLLATDYQGNTALHLCGHVDTIQFLVSNGLKIDICNHQGATPLVLAKRRGVNKDVIRLLESLEEQEVKGFNRGTHSKLETMQTAESESAMESHSLLNPNLQQGEGVLSSFRTTWQEFVED

相同性解析による交差性が予測される生物種
Full Name ankyrin repeat domain 46
Observed molecular weight 25 kDa
GenBank accession numberBC060843
Gene Symbol ANKRD46
Gene ID (NCBI) 157567
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ86W74
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ANKRD46 encodes a protein containing multiple ankyrin repeats. Ankyrin domains function in protein-protein interactions in a variety of cellular processes. ANKRD46 also known as ankyrin repeat small protein (ANK-S), is a 228-amino acid single-pass membrane protein(PMID: 21219636).

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