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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANKRD46 Polyclonal antibody (27568-1-AP)

    WB analysis using 27568-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27568-1-AP (ANKRD46 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ANKRD46 Polyclonal antibody (27568-1-AP)

    IF Staining of HEK-293 using 27568-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ANKRD46 antibody (27568-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ANKRD46 Polyclonal antibody

ANKRD46 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 27568-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Ankyrin repeat domain-containing protein 46, ankyrin repeat domain 46, ANK-S

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27568-1-AP targets ANKRD46 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26623

Product name: Recombinant human ANKRD46 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-189 aa of BC060843

Sequence: MSYVFVNDSSQTNVPLLQACIDGDFNYSKRLLESGFDPNIRDSRGRTGLHLAAARGNVDICQLLHKFGADLLATDYQGNTALHLCGHVDTIQFLVSNGLKIDICNHQGATPLVLAKRRGVNKDVIRLLESLEEQEVKGFNRGTHSKLETMQTAESESAMESHSLLNPNLQQGEGVLSSFRTTWQEFVED

相同性解析による交差性が予測される生物種
Full Name ankyrin repeat domain 46
Observed molecular weight 25 kDa
GenBank accession numberBC060843
Gene Symbol ANKRD46
Gene ID (NCBI) 157567
RRIDAB_3669610
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86W74
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANKRD46 encodes a protein containing multiple ankyrin repeats. Ankyrin domains function in protein-protein interactions in a variety of cellular processes. ANKRD46 also known as ankyrin repeat small protein (ANK-S), is a 228-amino acid single-pass membrane protein(PMID: 21219636).

Protocols

Product Specific Protocols
IF protocol for ANKRD46 antibody 27568-1-APDownload protocol
WB protocol for ANKRD46 antibody 27568-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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