Validation Data Gallery
Tested Applications
| Positive IP detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25765-1-AP targets ANKRD40 in IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22731 Product name: Recombinant human ANKRD40 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-111 aa of BC005853 Sequence: MQELVLKVRIQNPSLRENDFIEIELDRQELTYQELLRVCCCELGVNPDQVEKIRKLPNTLLRKDKDVARLQDFQELELVLMISENNFLFRNAASTLTERPCYNRRASKLTY 相同性解析による交差性が予測される生物種 |
| Full Name | ankyrin repeat domain 40 |
| Calculated molecular weight | 368 aa, 41 kDa |
| Observed molecular weight | 41 kDa |
| GenBank accession number | BC005853 |
| Gene Symbol | ANKRD40 |
| Gene ID (NCBI) | 91369 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6AI12 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ANKRD40 (ankyrin repeat domain 40) is a protein-coding gene conserved across mammals, including humans, mice, rats, and whales. The encoded protein contains ankyrin repeat domains, which are structural motifs typically involved in protein-protein interactions. In humans, ANKRD40 is located on chromosome 17 (17q21.33) and is ubiquitously expressed, with higher levels in tissues like the brain and adipose. ANKRD40 implicated in DNA replication, cell adhesion and migration; its expression correlates with tumor progression, yet precise molecular mechanisms remain undefined.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for ANKRD40 antibody 25765-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |