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Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using ANKRD33 Polyclonal antibody (25202-1-AP)

    WB analysis of Y79 using 25202-1-AP (same clone as 25202-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 25202-1-AP (ANKRD33 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25202-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using ANKRD33 Polyclonal antibody (25202-1-AP)

    IHC staining of human testis using 25202-1-AP (same clone as 25202-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25202-1-AP (ANKRD33 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25202-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using ANKRD33 Polyclonal antibody (25202-1-AP)

    IHC staining of human testis using 25202-1-AP (same clone as 25202-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25202-1-AP (ANKRD33 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25202-1-PBS in a different storage buffer formulation.

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ANKRD33 Polyclonal antibody, PBS Only

ANKRD33 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25202-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25202-1-PBS targets ANKRD33 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKRD33 fusion protein Ag18582 相同性解析による交差性が予測される生物種
Full Name ankyrin repeat domain 33
Calculated molecular weight 452 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC121153
Gene Symbol ANKRD33
Gene ID (NCBI) 341405
RRIDAB_2879956
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z3H0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Ankyrin repeat domain 33 (ANKRD33), also named as C12orf7, belongs to the ankyrin repeat family and is highly expressed in retina. ANKRD33 exists as two isoforms. The isoform 1 molecular weight is 29kDa and isoform 2 is 49kDa. The function of ANKRD33 is still non-known.