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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ANKRD26 Polyclonal antibody

ANKRD26 Polyclonal Antibody for IF, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20035-1-AP

Synonyms

ANKRD26, ankyrin repeat domain 26, KIAA1074

Validation Data Gallery

Applications:
  • IP experiment of HeLa cells using 20035-1-AP

    IP experiment of HeLa cells using 20035-1-AP

    IP result of anti-ANKRD26 (20035-1-AP for IP and Detection).

  • IHC staining of human breast cancer using 20035-1-AP

    IHC staining of human breast cancer using 20035-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20035-1-AP (ANKRD26 Antibody) at dilution of 1:50.

  • IHC staining of human colon using 20035-1-AP

    IHC staining of human colon using 20035-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 20035-1-AP (ANKRD26 Antibody) at dilution of 1:50.

  • IF Staining of HeLa using 20035-1-AP

    IF Staining of HeLa using 20035-1-AP

    Immunofluorescent analysis of HeLa cells using 20035-1-AP (ANKRD26 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

20035-1-AP targets ANKRD26 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ankyrin repeat domain 26
Calculated molecular weight 196 kDa
GenBank accession numberNM_014915
Gene symbol ANKRD26
Gene ID (NCBI) 22852
RRIDAB_11183053
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANKRD26 also named as KIAA1074. The antibody recognizes the C-term of ANKRD26.

Protocols

Product Specific Protocols
IHC protocol for ANKRD26 antibody 20035-1-APDownload protocol
IF protocol for ANKRD26 antibody 20035-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell

Transgenerational inheritance of acquired epigenetic signatures at CpG islands in mice

Authors - Yuta Takahashi
View Article