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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANKFY1 Polyclonal antibody (24890-1-AP)

    WB analysis using 24890-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 24890-1-AP (ANKFY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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ANKFY1 Polyclonal antibody

ANKFY1 Polyclonal Antibody for WB, ELISA
Cat No. 24890-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ANKFY1, ANKHZN, KIAA1255, ZFYVE14

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24890-1-AP targets ANKFY1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKFY1 fusion protein Ag19267 相同性解析による交差性が予測される生物種
Full Name ankyrin repeat and FYVE domain containing 1
Calculated molecular weight 1169 aa, 129 kDa
Observed molecular weight 125-135 kDa
GenBank accession numberBC152991
Gene Symbol ANKFY1
Gene ID (NCBI) 51479
RRIDAB_3669459
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2R3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANKFY1 also named as ANKHZN or Ankyrin repeats hooked to a zinc finger motif is a 1169 amino acid protein, which is a cytoplasm protein on embryonic stem cells. ANKFY1 protein is ubiquitously expressed in a spatiotemporal specific manner and is located on endosomes. ANKFY1 is expressed at various levels in human tissues and also present in both membrane and soluble fractions obtained on subcellular fractionation. ANKFY1 is a single copy gene consisting of predicted 25 exons in the human genome, and has been mapped to human chromosome 17p13.

Protocols

Product Specific Protocols
WB protocol for ANKFY1 antibody 24890-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Neural Regen Res

Ankfy1 is dispensable for neural stem/precursor cell development.

Authors - Chao Weng
View Article