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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using ANK3 Polyclonal antibody (27766-1-AP)

    IHC staining of mouse brain using 27766-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ANK3 Polyclonal antibody (27766-1-AP)

    IHC staining of mouse brain using 27766-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ANK3 Polyclonal antibody (27766-1-AP)

    IHC staining of mouse kidney using 27766-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ANK3 Polyclonal antibody (27766-1-AP)

    IHC staining of mouse kidney using 27766-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ANK3 Polyclonal antibody (27766-1-AP)

    IHC staining of human kidney using 27766-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ANK3 Polyclonal antibody (27766-1-AP)

    IHC staining of human kidney using 27766-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using ANK3 Polyclonal antibody (27766-1-AP)

    IF Staining of mouse brain using 27766-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ANK3 antibody (27766-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using ANK3 Polyclonal antibody (27766-1-AP)

    IF Staining of mouse brain using 27766-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ANK3 antibody (27766-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using ANK3 Polyclonal antibody (27766-1-AP)

    IF Staining of mouse brain using 27766-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using ANK3 antibody (27766-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ANK3 Polyclonal antibody

ANK3 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 27766-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Synonyms

ANK 3, Ankyrin 3, ANKYRIN G, Ankyrin3, ANKYRING

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse brain tissue, human kidney tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27766-1-AP targets ANK3 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26948

Product name: Recombinant human ANK3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 3668-3870 aa of NM_001149

Sequence: METNLERNVETPTVEPNPSIPTSGECQEGTSSSGSLEKSAAATNTSKVDPKLRTPIKMGISASTMTMKKEGPGEITDKIEAVMTSCQGLENETITMISNTANSQMGVRPHEKHDFQKDNFNNNNNLDSSTIQTDNIMSNIVLTEHSAPTCTTEKDNPVKVSSGKKTGVLQGHCVRDKQKVLGEQQKTKELIGIRQKSKLPIKAT

相同性解析による交差性が予測される生物種
Full Name ankyrin 3, node of Ranvier (ankyrin G)
Calculated molecular weight 480 kDa
Observed molecular weight 204 kDa
GenBank accession numberNM_001149
Gene Symbol ANK3
Gene ID (NCBI) 288
RRIDAB_2880966
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12955
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for ANK3 antibody 27766-1-APDownload protocol
IF protocol for ANK3 antibody 27766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols