Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, mouse liver tissue |
| Positive IHC detected in | human stomach cancer tissue, human lymphoma tissue, mouse cerebellum tissue, rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23938-1-AP targets ANK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21041 Product name: Recombinant human ANK1 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-155 aa of BC030957 Sequence: MWTFVTQLLVTLVLLSFFLVSCQNVMHIVRGSLCFVLKHIHQELDKELGESEDLSDDEETISTRVVRRRVFLKGNEFQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQIDLSSADAAQEHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ 相同性解析による交差性が予測される生物種 |
| Full Name | ankyrin 1, erythrocytic |
| Calculated molecular weight | 155 aa, 18 kDa |
| Observed molecular weight | 190-250 kDa |
| GenBank accession number | BC030957 |
| Gene Symbol | ANK1 |
| Gene ID (NCBI) | 286 |
| RRID | AB_3085717 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | P16157-17 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ankyrin-1 (ANK1) is also named as Ankyrin-R and Erythrocyte ankyrin. The stability and shape of the erythrocyte membrane is provided by the ankyrin-1 complex (PMID:18440852). ANK1 can attache integral membrane proteins to cytoskeletal elements. And it binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin.(PMID:12456646). This target recognizes the C-terminal of Ankyrin-1 and also recognizes ankyrin-1 isoform 5 (206 kDa). It can identify isoforms such as Er5, Er6, Er11, Er12, Er13, Er14, Er16, Mu17, Mu18, Mu19, Mu20, 22, and 23.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ANK1 antibody 23938-1-AP | Download protocol |
| WB protocol for ANK1 antibody 23938-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |