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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using ANGPTL4 Polyclonal antibody (31497-1-AP)

    WB analysis of HEK-293 using 31497-1-AP

    WB result of ANGPTL4 antibody (31497-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ANGPTL4 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using ANGPTL4 Polyclonal antibody (31497-1-AP)

    WB analysis using 31497-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31497-1-AP (ANGPTL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using ANGPTL4 Polyclonal antibody (31497-1-AP)

    IHC staining of human placenta using 31497-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31497-1-AP (ANGPTL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using ANGPTL4 Polyclonal antibody (31497-1-AP)

    IHC staining of human placenta using 31497-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31497-1-AP (ANGPTL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ANGPTL4 Polyclonal antibody (31497-1-AP)

    IF Staining of HEK-293 using 31497-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ANGPTL4 antibody (31497-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using ANGPTL4 Polyclonal antibody (31497-1-AP)

    IF Staining of NIH/3T3 using 31497-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using ANGPTL4 antibody (31497-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using ANGPTL4 Polyclonal antibody (31497-1-AP)

    IF Staining of HT-29 using 31497-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using ANGPTL4 antibody (31497-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ANGPTL4 Polyclonal antibody

ANGPTL4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31497-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

angiopoietin like 4, Angiopoietin like protein 4, Angiopoietin related protein 4, Angiopoietin-like protein 4, Angiopoietin-related protein 4

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, HEK-293 cells, rat heart tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells, NIH/3T3 cells, HT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31497-1-AP targets ANGPTL4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag31212

Product name: Recombinant human ANGPTL4 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 26-200 aa of BC023647

Sequence: GPVQSKSPRFASWDEMNVLAHGLLQLGQGLREHAERTRSQLSALERRLSACGSACQGTEGSTDLPLAPESRVDPEVLHSLQTQLKAQNSRIQQLFHKVAQQQRHLEKQHLRIQHLQSQFGLLDHKHLDHEVAKPARRKRLPEMAQPVDPAHNVSRLHRLPRDCQELFQVGERQSG

相同性解析による交差性が予測される生物種
Full Name angiopoietin-like 4
Calculated molecular weight 45 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC023647
Gene Symbol ANGPTL4
Gene ID (NCBI) 51129
RRIDAB_3742439
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9BY76
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Angiopoietin-like protein 4 (ANGPTL4), a member of the angiopoietin-like protein subfamily, is a secretory protein that functions as an important modulator of glucose and lipid metabolism. ANGPTL4 inhibits lipoprotein lipase (LPL)-dependent lipolysis thereby limiting the uptake of free fatty acids. Overexpression of ANGPTL4 results in hypertriglyceridemia, while ANGPTL4 deficiency suppresses foam formation in macrophages and protects against atherosclerosis. ANGPTL4 may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes.

Protocols

Product Specific Protocols
IF protocol for ANGPTL4 antibody 31497-1-APDownload protocol
IHC protocol for ANGPTL4 antibody 31497-1-APDownload protocol
WB protocol for ANGPTL4 antibody 31497-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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