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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANGPTL1 Polyclonal antibody (26934-1-AP)

    WB analysis using 26934-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26934-1-AP (ANGPTL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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ANGPTL1 Polyclonal antibody

ANGPTL1 Polyclonal Antibody for WB, ELISA
Cat No. 26934-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inJurkat cells, MCF-7 cells, mouse liver tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26934-1-AP targets ANGPTL1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANGPTL1 fusion protein Ag25529 相同性解析による交差性が予測される生物種
Full Name angiopoietin-like 1
Calculated molecular weight 55 kDa
Observed molecular weight64 -70 kDa
GenBank accession numberBC050640
Gene Symbol ANGPTL1
Gene ID (NCBI) 9068
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95841
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Angiopoietin-related protein 1 (ANGPTL1) also known as angiopoietin-3 (ANG-3), is a secreted glycoprotein that structurally resembles angiogenin and is involved in the regulation of angiogenesis. ANGPTL1 is down-regulated in various cancers, including colorectal cancer (CRC), and its expression is inversely correlated with metastasis and poor clinical outcomes. ANGPTL1 has been shown to suppress angiogenesis, cancer invasion, metastasis, and cancer progression. The predicted molecular weight of ANGPTL1 is 57 kDa, but the literature reports that the detected molecular weight of ANGPTL1 is around 68 kDa (PMID: 15284220 ).

Protocols

Product Specific Protocols
WB protocol for ANGPTL1 antibody 26934-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols