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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANAPC5 Monoclonal antibody (67348-1-Ig)

    WB analysis using 67348-1-Ig (same clone as 67348-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67348-1-Ig (ANAPC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67348-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ANAPC5 Monoclonal antibody (67348-1-Ig)

    IHC staining of human breast cancer using 67348-1-Ig (same clone as 67348-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67348-1-Ig (ANAPC5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67348-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ANAPC5 Monoclonal antibody (67348-1-Ig)

    IHC staining of human breast cancer using 67348-1-Ig (same clone as 67348-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67348-1-Ig (ANAPC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67348-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using ANAPC5 Monoclonal antibody (67348-1-Ig)

    IF Staining of human breast cancer using 67348-1-Ig (same clone as 67348-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ANAPC5 antibody (67348-1-Ig, Clone: 2A7G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67348-1-PBS in a different storage buffer formulation.

ANAPC5 Monoclonal antibody, PBS Only

ANAPC5 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67348-1-PBS
Clone No.2A7G5

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

ANAPC5, APC5, Cyclosome subunit 5

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67348-1-PBS targets ANAPC5 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7409

Product name: Recombinant human ANAPC5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-343 aa of BC001081

Sequence: MASVHESLYFNPMMTNGVVHANVFGIKDWVTPYKIAVLVLLNEMSRTGEGAVSLMERRRLNQLLLPLLQGPDITLSKLYKLIEESCPQLANSVQIRIKLMAEGELKDMEQFFDDLSDSFSGTEPEVHKTSVVGLFLRHMILAYSKLSFSQVFKLYTALQQYFQNGEKKTVEDADMELTSRDEGERKMEKEELDVSVREEEVSCSGPLSQKQAEFFLSQQASLLKNDETKALTPASLQKELNNLLKFNPDFAEAHYLSYLNNLRVQDVFSSTHSLLHYFDRLILTGAESKSNGEEGYGRSLRYAALNLAALHCRFGHYQQAELALQEAIRIAQESNDHVCLQHC

相同性解析による交差性が予測される生物種
Full Name anaphase promoting complex subunit 5
Calculated molecular weight 85 kDa
Observed molecular weight 85 kDa, 72 kDa
GenBank accession numberBC001081
Gene Symbol ANAPC5
Gene ID (NCBI) 51433
RRIDAB_2882606
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9UJX4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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