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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using ANAPC5 Polyclonal antibody (14926-1-AP)

    WB analysis of mouse lung using 14926-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 14926-1-AP (ANAPC5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using ANAPC5 Polyclonal antibody (14926-1-AP)

    WB analysis of mouse lung using 14926-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 14926-1-AP (ANAPC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using ANAPC5 Polyclonal antibody (14926-1-AP)

    WB analysis of BxPC-3 using 14926-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14926-1-AP (ANAPC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ANAPC5 Polyclonal antibody (14926-1-AP)

    IHC staining of human pancreas cancer using 14926-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14926-1-AP (ANAPC5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ANAPC5 Polyclonal antibody (14926-1-AP)

    IHC staining of human pancreas cancer using 14926-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14926-1-AP (ANAPC5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ANAPC5 Polyclonal antibody

ANAPC5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14926-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Anaphase-promoting complex subunit 5, APC5, Cyclosome subunit 5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse lung tissue, BxPC-3 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14926-1-AP targets ANAPC5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6688

Product name: Recombinant human ANAPC5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-343 aa of BC001081

Sequence: MASVHESLYFNPMMTNGVVHANVFGIKDWVTPYKIAVLVLLNEMSRTGEGAVSLMERRRLNQLLLPLLQGPDITLSKLYKLIEESCPQLANSVQIRIKLMAEGELKDMEQFFDDLSDSFSGTEPEVHKTSVVGLFLRHMILAYSKLSFSQVFKLYTALQQYFQNGEKKTVEDADMELTSRDEGERKMEKEELDVSVREEEVSCSGPLSQKQAEFFLSQQASLLKNDETKALTPASLQKELNNLLKFNPDFAEAHYLSYLNNLRVQDVFSSTHSLLHYFDRLILTGAESKSNGEEGYGRSLRYAALNLAALHCRFGHYQQAELALQEAIRIAQESNDHVCLQHC

相同性解析による交差性が予測される生物種
Full Name anaphase promoting complex subunit 5
Calculated molecular weight 85 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC001081
Gene Symbol ANAPC5
Gene ID (NCBI) 51433
RRIDAB_2227073
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJX4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ANAPC5 antibody 14926-1-APDownload protocol
IHC protocol for ANAPC5 antibody 14926-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols