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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ANAPC10 Recombinant antibody (83570-4-RR)

    IF Staining of HeLa using 83570-4-RR (same clone as 83570-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ANAPC10 antibody (83570-4-RR, Clone: 240492E7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83570-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83570-4-RR

    Affinity and Kinetic Characterization of 83570-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83570-4-RR against Human ANAPC10 were performed. The affinity constant is 0.141 nM.

ANAPC10 Recombinant monoclonal antibody, PBS Only

ANAPC10 Uni-rAb® Recombinant Antibody for IF/ICC, ELISA
Cat No. 83570-4-PBS
Clone No.240492E7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

DOC1, APC10, Anaphase-promoting complex subunit 10, ANAPC 10, 240492E7

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83570-4-PBS targets ANAPC10 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8487

Product name: Recombinant human ANAPC10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-185 aa of BC005217

Sequence: MTTPNKTPPGADPKQLERTGTVREIGSQAVWSLSSCKPGFGVDQLRDDNLETYWQSDGSQPHLVNIQFRRKTTVKTLCIYADYKSDESYTPSKISVRVGNNFHNLQEIRQLELVEPSGWIHVPLTDNHKKPTRTFMIQIAVLANHQNGRDTHMRQIKIYTPVEESSIGKFPRCTTIDFMMYRSIR

相同性解析による交差性が予測される生物種
Full Name anaphase promoting complex subunit 10
Calculated molecular weight 185 aa, 21 kDa
GenBank accession numberBC005217
Gene Symbol ANAPC10
Gene ID (NCBI) 10393
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9UM13
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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