Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
24463-1-AP targets AMTN in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18439 Product name: Recombinant human AMTN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-209 aa of BC121817 Sequence: MRSTILLFCLLGSTRSLPQLKPALGLPPTKLAPDQGTLPNQQQSNQVFPSLSLIPLTQMLTLGPDLHLLNPAAGMTPGTQTHPLTLGGLNVQQQLHPHVLPIFVTQLGAQGTILSSEELPQIFTSLIIHSLFPGGILPTSQAGANPDVQDGSLPAGGAGVNPATQGTPAGRLPTPSGTDDDFAVTTPAGIQRSTHAIEEATTESANGIQ 相同性解析による交差性が予測される生物種 |
| Full Name | amelotin |
| Calculated molecular weight | 209 aa, 22 kDa |
| GenBank accession number | BC121817 |
| Gene Symbol | AMTN |
| Gene ID (NCBI) | 401138 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6UX39 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Amelotin, also named as AMTN, is a novel factor produced by ameloblasts that plays a critical role in the formation of dental enamel.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AMTN antibody 24463-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Dev Dyn Maturation stage enamel defects in Odontogenesis-associated phosphoprotein (Odaph) deficient mice. | ||
Oral Dis Epithelium-Specific Runx2 knockout mice display junctional epithelium and alveolar bone defects. | ||
J Mol Histol Expression and localization of amelotin, laminin γ2 and odontogenesis-associated phosphoprotein (ODAPH) on the basal lamina and junctional epithelium. |
