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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Aminomethyltransferase Polyclonal antibody

Aminomethyltransferase Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 10633-1-AP

Synonyms

Aminomethyltransferase, AMT, GCE, GCST, GCVT, NKH

Validation Data Gallery

Applications:
  • WB analysis using 10633-1-AP

    WB analysis using 10633-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10633-1-AP (Aminomethyltransferase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 10633-1-AP

    IHC staining of mouse liver using 10633-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10633-1-AP (Aminomethyltransferase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse kidney tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10633-1-AP targets Aminomethyltransferase in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Aminomethyltransferase fusion protein Ag0990 相同性解析による交差性が予測される生物種
Full Name aminomethyltransferase
Calculated molecular weight 44 kDa
Observed molecular weight40 kDa
GenBank accession numberBC007546
Gene symbol AMT
Gene ID (NCBI) 275
RRIDAB_2305496
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Aminomethyltransferase antibody 10633-1-APDownload protocol
IHC protocol for Aminomethyltransferase antibody 10633-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

BMC Complement Med Ther

Vincetoxicum arnottianum modulates motility features and metastatic marker expression in pediatric rhabdomyosarcoma by stabilizing the actin cytoskeleton.

Authors - Anna Adamus
View Article