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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using AMPK Alpha 2 Polyclonal antibody (18167-1-AP)

    WB analysis of HEK-293 using 18167-1-AP (same clone as 18167-1-PBS)

    WB result of AMPK alpha 2 antibody (18167-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-AMPK alpha 2 transfected HEK-293 cells. This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AMPK Alpha 2 Polyclonal antibody (18167-1-AP)

    WB analysis using 18167-1-AP (same clone as 18167-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18167-1-AP (AMPK alpha 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat ovary tissue using AMPK Alpha 2 Polyclonal antibody (18167-1-AP)

    WB analysis of rat ovary using 18167-1-AP (same clone as 18167-1-PBS)

    rat ovary tissue were subjected to SDS PAGE followed by western blot with 18167-1-AP (AMPK alpha 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using AMPK Alpha 2 Polyclonal antibody (18167-1-AP)

    WB analysis of HEK-293 using 18167-1-AP (same clone as 18167-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18167-1-AP (AMPK alpha 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using AMPK Alpha 2 Polyclonal antibody (18167-1-AP)

    WB analysis of human skeletal muscle using 18167-1-AP (same clone as 18167-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18167-1-AP (AMPK alpha 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AMPK Alpha 2 Polyclonal antibody (18167-1-AP)

    WB analysis using 18167-1-AP (same clone as 18167-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18167-1-AP (AMPK alpha 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using AMPK Alpha 2 Polyclonal antibody (18167-1-AP)

    WB analysis of HEK-293 using 18167-1-AP (same clone as 18167-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18167-1-AP (AMPK alpha 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using AMPK Alpha 2 Polyclonal antibody (18167-1-AP)

    IP experiment of mouse skeletal muscle using 18167-1-AP (same clone as 18167-1-PBS)

    IP result of anti-AMPK Alpha 2 (IP:18167-1-AP, 4ug; Detection:18167-1-AP 1:1000) with mouse skeletal muscle tissue lysate 3600ug. This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AMPK Alpha 2 Polyclonal antibody (18167-1-AP)

    IHC staining of human breast cancer using 18167-1-AP (same clone as 18167-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18167-1-AP (AMPK alpha 2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AMPK Alpha 2 Polyclonal antibody (18167-1-AP)

    IHC staining of human breast cancer using 18167-1-AP (same clone as 18167-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18167-1-AP (AMPK alpha 2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using AMPK Alpha 2 Polyclonal antibody (18167-1-AP)

    IHC staining of mouse skeletal muscle using 18167-1-AP (same clone as 18167-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18167-1-AP (AMPK alpha 2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using AMPK Alpha 2 Polyclonal antibody (18167-1-AP)

    IHC staining of mouse skeletal muscle using 18167-1-AP (same clone as 18167-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18167-1-AP (AMPK alpha 2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using AMPK Alpha 2 Polyclonal antibody (18167-1-AP)

    IF Staining of MCF-7 using 18167-1-AP (same clone as 18167-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using AMPK Alpha 2 antibody (18167-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

AMPK Alpha 2 Polyclonal antibody, PBS Only

AMPK Alpha 2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 18167-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

PRKAA2, 5'-AMP-activated protein kinase catalytic subunit alpha-2, ACACA kinase, Acetyl-CoA carboxylase kinase, AMPK

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18167-1-PBS targets AMPK Alpha 2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12796

Product name: Recombinant human PRKAA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 215-552 aa of BC069680

Sequence: DDEHVPTLFKKIRGGVFYIPEYLNRSVATLLMHMLQVDPLKRATIKDIREHEWFKQDLPSYLFPEDPSYDANVIDDEAVKEVCEKFECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIMNQASEFYLASSPPSGSFMDDSAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKPKSLAVKKAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVVNAYHLRVRRKNPVTGNYVKMSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRSSFDSTTAESHSLSGSLTGSLTGSTLSSVSPRLGSHTMDFFEMCASLITTLAR

相同性解析による交差性が予測される生物種
Full Name protein kinase, AMP-activated, alpha 2 catalytic subunit
Calculated molecular weight 552 aa, 62 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC069680
Gene Symbol AMPK Alpha 2
Gene ID (NCBI) 5563
RRIDAB_10695046
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP54646
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PRKAA2(protein kinase, AMP-activated, alpha 2 catalytic subunit), also named as AMPKA2, AMPK, PRKAA, AMPK2, belongs to the CAMK Ser/Thr protein kinase family and SNF1 subfamily. PRKAA2 is an αβγ heterotrimer that is activated by low cellular energy status, such as decreases in both the ATP/AMP ratio and the phosphocreatine content and it is a glycogen synthase kinase, phosphorylating Ser7 at the NH2 terminus, which decreases glycogen synthase activity (PMID:14532170). The protein can be ubiquitinated (PMID:21224036).

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