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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using AMPK Alpha 1 Monoclonal antibody (66536-1-Ig)

    WB analysis of HEK-293 using 66536-1-Ig (same clone as 66536-1-PBS)

    WB result of AMPK alpha antibody (66536-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-AMPK alpha transfected HEK-293 cells. This data was developed using the same antibody clone with 66536-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AMPK Alpha 1 Monoclonal antibody (66536-1-Ig)

    WB analysis using 66536-1-Ig (same clone as 66536-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66536-1-Ig (AMPK alpha antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66536-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AMPK Alpha 1 Monoclonal antibody (66536-1-Ig)

    WB analysis using 66536-1-Ig (same clone as 66536-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66536-1-Ig (AMPK alpha antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66536-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using AMPK Alpha 1 Monoclonal antibody (66536-1-Ig)

    WB analysis of Jurkat using 66536-1-Ig (same clone as 66536-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66536-1-Ig (AMPK alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66536-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using AMPK Alpha 1 Monoclonal antibody (66536-1-Ig)

    WB analysis of HSC-T6 using 66536-1-Ig (same clone as 66536-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66536-1-Ig (AMPK alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66536-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using AMPK Alpha 1 Monoclonal antibody (66536-1-Ig)

    WB analysis of NIH/3T3 using 66536-1-Ig (same clone as 66536-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66536-1-Ig (AMPK alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66536-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AMPK Alpha 1 Monoclonal antibody (66536-1-Ig)

    WB analysis using 66536-1-Ig (same clone as 66536-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66536-1-Ig (AMPK alpha antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66536-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AMPK Alpha 1 Monoclonal antibody (66536-1-Ig)

    IHC staining of human breast cancer using 66536-1-Ig (same clone as 66536-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66536-1-Ig (AMPK alpha antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66536-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AMPK Alpha 1 Monoclonal antibody (66536-1-Ig)

    IHC staining of human breast cancer using 66536-1-Ig (same clone as 66536-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66536-1-Ig (AMPK alpha antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66536-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using AMPK Alpha 1 Monoclonal antibody (66536-1-Ig)

    IHC staining of human prostate cancer using 66536-1-Ig (same clone as 66536-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66536-1-Ig (AMPK alpha antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66536-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using AMPK Alpha 1 Monoclonal antibody (66536-1-Ig)

    IHC staining of human prostate cancer using 66536-1-Ig (same clone as 66536-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66536-1-Ig (AMPK alpha antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66536-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

AMPK Alpha 1 Monoclonal antibody, PBS Only

AMPK Alpha 1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66536-1-PBS
Clone No.1H8E1

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

AMPK, AMPK alpha, AMPK Alpha 1, AMPK subunit alpha 1, AMPK1, AMPKa1, PRKAA1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66536-1-PBS targets AMPK Alpha 1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag24744

Product name: Recombinant human AMPK alpha protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-207 aa of BC012622

Sequence: MATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRDCNIIRILTSQFTNYQH

相同性解析による交差性が予測される生物種
Full Name protein kinase, AMP-activated, alpha 1 catalytic subunit
Calculated molecular weight 63 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC012622
Gene Symbol AMPK Alpha 1
Gene ID (NCBI) 5562
RRIDAB_2881899
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ13131
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.

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