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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using AMPD1-Specific Polyclonal antibody (19780-1-AP)

    WB analysis of mouse skeletal muscle using 19780-1-AP (same clone as 19780-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19780-1-AP (AMPD1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19780-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using AMPD1-Specific Polyclonal antibody (19780-1-AP)

    WB analysis of mouse skeletal muscle using 19780-1-AP (same clone as 19780-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19780-1-AP (AMPD1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19780-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using AMPD1-Specific Polyclonal antibody (19780-1-AP)

    IP experiment of mouse skeletal muscle using 19780-1-AP (same clone as 19780-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19780-1-AP (AMPD1-Specific antibody) at dilution of 1:300. This data was developed using the same antibody clone with 19780-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using AMPD1-Specific Polyclonal antibody (19780-1-AP)

    IHC staining of mouse skeletal muscle using 19780-1-AP (same clone as 19780-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19780-1-AP (AMPD1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19780-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using AMPD1-Specific Polyclonal antibody (19780-1-AP)

    IHC staining of mouse skeletal muscle using 19780-1-AP (same clone as 19780-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19780-1-AP (AMPD1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19780-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

AMPD1-Specific Polyclonal antibody, PBS Only

AMPD1-Specific Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 19780-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19780-1-PBS targets AMPD1-Specific in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name adenosine monophosphate deaminase 1 (isoform M)
Calculated molecular weight 87 kDa
Observed molecular weight 75-83 kDa
GenBank accession numberNM_000036
Gene Symbol AMPD1
Gene ID (NCBI) 270
RRIDAB_10644281
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP23109
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

AMPD1, also named as MAD and MADA, belongs to the adenosine and AMP deaminases family. AMP deaminase plays a critical role in energy metabolism. It catalyzes the reaction: AMP + H2O = IMP + NH3. Defects in AMPD1 are the cause of adenosine monophosphate deaminase deficiency muscle type (AMPDDM) which is a metabolic disorder resulting in exercise-related myopathy. The antibody is specific to AMPD1.

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