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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AMOTL2 Monoclonal antibody (68401-1-Ig)

    WB analysis using 68401-1-Ig (same clone as 68401-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68401-1-Ig (AMOTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68401-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of DU 145 cells using AMOTL2 Monoclonal antibody (68401-1-Ig)

    WB analysis of DU 145 using 68401-1-Ig (same clone as 68401-1-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 68401-1-Ig (AMOTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68401-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Saos-2 cells using AMOTL2 Monoclonal antibody (68401-1-Ig)

    WB analysis of Saos-2 using 68401-1-Ig (same clone as 68401-1-PBS)

    Saos-2 cells were subjected to SDS PAGE followed by western blot with 68401-1-Ig (AMOTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68401-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCI-H1299 cells using AMOTL2 Monoclonal antibody (68401-1-Ig)

    WB analysis of NCI-H1299 using 68401-1-Ig (same clone as 68401-1-PBS)

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 68401-1-Ig (AMOTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68401-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using AMOTL2 Monoclonal antibody (68401-1-Ig)

    IF Staining of A549 using 68401-1-Ig (same clone as 68401-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using AMOTL2 antibody (68401-1-Ig, Clone: 2C4A4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68401-1-PBS in a different storage buffer formulation.

AMOTL2 Monoclonal antibody, PBS Only

AMOTL2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68401-1-PBS
Clone No.2C4A4

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

AMOTL2, angiomotin like 2, Angiomotin like protein 2, KIAA0989, LCCP, Leman coiled coil protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68401-1-PBS targets AMOTL2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag19971

Product name: Recombinant human AMOTL2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 340-466 aa of BC011454

Sequence: DPGKAIQGSLRPAKSVPSVFAAAAAGTQGWQGLSSSERQTADAPARLTTDRAPTEEPVVTAPPAAHAKHGSRDGSTQTEGPPDSTSTCLPPEPDSLLGCSSSQRAASLDSVATSRVQDLSDMVEILI

相同性解析による交差性が予測される生物種
Full Name angiomotin like 2
Calculated molecular weight 779 aa, 86 kDa
Observed molecular weight100 kDa
GenBank accession numberBC011454
Gene Symbol AMOTL2
Gene ID (NCBI) 51421
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9Y2J4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Angiomotin‑like 2 (AMOTL2) is a member of the motin family of angiostatin‑binding proteins. The motin family, also known as AMOTs, consists of three members: AMOT, AMOT-like 1 (AMOTL1) and AMOTL2. Members of the motin family are a type of adaptor proteins mainly distributed in the cytomembrane, cytoplasm or nucleus, and have a higher expression in the endothelial cells of capillaries and in larger vessels of the placenta. Human AmotL2 encodes two isoforms of a molecular mass of 100 kDa and 60 kDa.(PMID: 34036399,PMID: 25080976)

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