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Species Reactivity
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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AMN1 Polyclonal antibody

AMN1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14694-1-AP

Synonyms

AMN1, Protein AMN1 homolog

Validation Data Gallery

Applications:
  • WB analysis of human colon using 14694-1-AP

    WB analysis of human colon using 14694-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 14694-1-AP (AMN1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 14694-1-AP

    IHC staining of human colon cancer using 14694-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 14694-1-AP (AMN1 antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inhuman colon tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14694-1-AP targets AMN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMN1 fusion protein Ag6257 相同性解析による交差性が予測される生物種
Full Name antagonist of mitotic exit network 1 homolog (S. cerevisiae)
Calculated molecular weight 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC067906
Gene symbol AMN1
Gene ID (NCBI) 196394
RRIDAB_2226647
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for AMN1 antibody 14694-1-APDownload protocol
IHC protocol for AMN1 antibody 14694-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols