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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using AMDHD1 Recombinant antibody (86415-1-RR)

    WB analysis of mouse liver using 86415-1-RR (same clone as 86415-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 86415-1-RR (AMDHD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86415-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AMDHD1 Recombinant antibody (86415-1-RR)

    WB analysis using 86415-1-RR (same clone as 86415-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86415-1-RR (AMDHD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86415-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86415-1-RR

    Affinity and Kinetic Characterization of 86415-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86415-1-RR against Human AMDHD1 were performed. The affinity constant is 9.59 nM.

AMDHD1 Recombinant monoclonal antibody, PBS Only

AMDHD1 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 86415-1-PBS
Clone No.250819A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Amidohydrolase domain-containing protein 1, EC:3.5.2.7, HMFT1272

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86415-1-PBS targets AMDHD1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AMDHD1 fusion protein Ag15420 相同性解析による交差性が予測される生物種
Full Name amidohydrolase domain containing 1
Calculated molecular weight 426 aa, 47 kDa
Observed molecular weight45-50 kDa
GenBank accession numberBC029146
Gene Symbol AMDHD1
Gene ID (NCBI) 144193
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ96NU7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

AMDHD1 is a protein-coding gene that encodes the enzyme imidazolonepropionase. It is involved in the histidine catabolic process and has one potential isoform. The protein of AMDHD1 is located in the cytoplasm, and is expressed in all tissues, mainly in the liver, kidney and adrenal gland.