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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AMBN Polyclonal antibody (26501-1-AP)

    WB analysis using 26501-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26501-1-AP (AMBN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using AMBN Polyclonal antibody (26501-1-AP)

    IF Staining of HEK-293 using 26501-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using AMBN antibody (26501-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

AMBN Polyclonal antibody

AMBN Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26501-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Ameloblastin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26501-1-AP targets AMBN in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMBN fusion protein Ag24129 相同性解析による交差性が予測される生物種
Full Name ameloblastin (enamel matrix protein)
Calculated molecular weight 447 aa, 48 kDa
Observed molecular weight48-55 kDa
GenBank accession numberBC106931
Gene Symbol AMBN
Gene ID (NCBI) 258
RRIDAB_3669542
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NP70
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AMBN(Ameloblastin), the gene encodes the nonamelogenin enamel matrix protein ameloblastin. The encoded protein may be important in enamel matrix formation and mineralization. Mutations in this gene may be associated with dentinogenesis imperfect and autosomal dominant amylogenesis imperfect. It is expected to be located in the Vesicles, in addition localized to the Nucleoplasm, which is enriched in brain, and located at the Tomes processes of secretory ameloblasts and in the sheath space between rod-interrod enamel. The molecular weight of AMBN is 48 kDa. It also has phosphorylation modification.

Protocols

Product Specific Protocols
WB protocol for AMBN antibody 26501-1-APDownload protocol
IF protocol for AMBN antibody 26501-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols