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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using ALX3 Polyclonal antibody (22358-1-AP)

    WB analysis of mouse testis using 22358-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 22358-1-AP (ALX3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ALX3 Polyclonal antibody (22358-1-AP)

    WB analysis of HEK-293 using 22358-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22358-1-AP (ALX3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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ALX3 Polyclonal antibody

ALX3 Polyclonal Antibody for WB, ELISA
Cat No. 22358-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

ALX homeobox 3, ALX3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse testis tissue, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22358-1-AP targets ALX3 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALX3 fusion protein Ag17937 相同性解析による交差性が予測される生物種
Full Name ALX homeobox 3
Calculated molecular weight 343 aa, 37 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC112007
Gene Symbol ALX3
Gene ID (NCBI) 257
RRIDAB_2879088
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDO95076
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ALX3 is a transcriptional regulator with a possible role in patterning of mesoderm during development. It is a paired-class aristaless-like homeoprotein expressed in different tissues during embryonic development. We always got 70 kDa dimer form in our detection.

Protocols

Product Specific Protocols
WB protocol for ALX3 antibody 22358-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols