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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALR Recombinant antibody (84972-4-RR)

    WB analysis using 84972-4-RR (same clone as 84972-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84972-4-RR (GFER antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84972-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using ALR Recombinant antibody (84972-4-RR)

    FC experiment of HepG2 using 84972-4-RR (same clone as 84972-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ALR Recombinant antibody (84972-4-RR, Clone:242557G3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84972-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84972-4-RR

    Affinity and Kinetic Characterization of 84972-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84972-4-RR against Human ALR were performed. The affinity constant is 0.23 nM.

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ALR Recombinant antibody, PBS Only

ALR Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84972-4-PBS
Clone No.242557G3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

GFER, 242557G3, EC:1.8.3.2, ERV1, FAD-linked sulfhydryl oxidase ALR

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84972-4-PBS targets ALR in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ALR fusion protein Ag1840 相同性解析による交差性が予測される生物種
Full Name growth factor, augmenter of liver regeneration
Calculated molecular weight 15 kDa, 23 kDa
Observed molecular weight 23-25 kDa
GenBank accession numberBC028348
Gene Symbol GFER
Gene ID (NCBI) 2671
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55789
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GFER (FAD-linked sulfhydryl oxidase) is also named as ALR, HERV1, HPO. It plays an important role in the disulfide relay system (DRS) in human mitochondria. The GFER gene codes for 2 distinct isoforms that are probably synthesized from the same mRNA with the use of different initiation codons. The long isoform (205 amino acids, 23/21 kD) is located mainly in the mitochondrial intermembrane space and exists under nonreducing and nondenaturing conditions as a homodimer and a heterodimer. The shorter isoform (125 amino acids, 15 kD), which lacks 80 amino acids at its N terminus compared to the longer isoform, is present predominantly in the nucleus (PMID: 19409522, 24880092, 21152698).