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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using ALPPL2 Recombinant monoclonal antibody (84813-1-RR)

    WB analysis of human placenta using 84813-1-RR (same clone as 84813-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 84813-1-RR (ALPPL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84813-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using ALPPL2 Recombinant monoclonal antibody (84813-1-RR)

    IHC staining of human placenta using 84813-1-RR (same clone as 84813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84813-1-RR (ALPPL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84813-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using ALPPL2 Recombinant monoclonal antibody (84813-1-RR)

    IHC staining of human placenta using 84813-1-RR (same clone as 84813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84813-1-RR (ALPPL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84813-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using ALPPL2 Recombinant monoclonal antibody (84813-1-RR)

    IF Staining of human placenta using 84813-1-RR (same clone as 84813-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using ALPPL2 antibody (84813-1-RR, Clone: 241867A12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84813-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84813-1-RR

    Affinity and Kinetic Characterization of 84813-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84813-1-RR against Human ALPPL2 were performed. The affinity constant is below 1 pM.

ALPPL2 Recombinant monoclonal antibody, PBS Only

ALPPL2 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 84813-1-PBS
Clone No.241867A12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

241867A12, Alkaline phosphatase, germ cell type, Alkaline phosphatase, placental-like, ALP1, ALP-1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84813-1-PBS targets ALPPL2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag13360

Product name: Recombinant human ALPPL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 223-532 aa of BC014139

Sequence: RKYMFPMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDGETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPRAGTTDAAHPGPSVVPALLPLLAGTLLLLGTATAP

相同性解析による交差性が予測される生物種
Full Name alkaline phosphatase, placental-like 2
Calculated molecular weight 57 kDa
Observed molecular weight70 kDa
GenBank accession numberBC014139
Gene Symbol ALPPL2
Gene ID (NCBI) 251
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP10696
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ALPPL2 (alkaline phosphatase placental-like 2), a member of the human alkaline phosphatase family, could be a potential biomarker for early diagnosis of Pancreatic ductal adenocarcinoma.

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