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Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using ALPL Recombinant monoclonal antibody (82915-2-RR)

    WB analysis of L02 using 82915-2-RR (same clone as 82915-2-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 82915-2-RR (ALPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82915-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using ALPL Recombinant monoclonal antibody (82915-2-RR)

    FC experiment of HeLa using 82915-2-RR (same clone as 82915-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ALPL Recombinant antibody (82915-2-RR, Clone:230212D5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82915-2-PBS in a different storage buffer formulation.

ALPL Recombinant monoclonal antibody, PBS Only

ALPL Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 82915-2-PBS
Clone No.230212D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

Alkaline phosphatase, 230212D5, AKP2, Alkaline phosphatase liver/bone/kidney isozyme, Alkaline phosphatase tissue nonspecific isozyme

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82915-2-PBS targets ALPL in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1664

Product name: Recombinant human ALPL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 23-330 aa of BC021289

Sequence: EKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLL

相同性解析による交差性が予測される生物種
Full Name alkaline phosphatase, liver/bone/kidney
Calculated molecular weight 57 kDa
GenBank accession numberBC021289
Gene Symbol ALPL
Gene ID (NCBI) 249
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP05186
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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