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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALPK1 Monoclonal antibody (67689-1-Ig)

    WB analysis using 67689-1-Ig (same clone as 67689-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67689-1-Ig (ALPK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67689-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using ALPK1 Monoclonal antibody (67689-1-Ig)

    IHC staining of human heart using 67689-1-Ig (same clone as 67689-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67689-1-Ig (ALPK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67689-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using ALPK1 Monoclonal antibody (67689-1-Ig)

    IHC staining of human heart using 67689-1-Ig (same clone as 67689-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67689-1-Ig (ALPK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67689-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using ALPK1 Monoclonal antibody (67689-1-Ig)

    IF Staining of THP-1 using 67689-1-Ig (same clone as 67689-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using ALPK1 antibody (67689-1-Ig, Clone: 2B8B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67689-1-PBS in a different storage buffer formulation.

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ALPK1 Monoclonal antibody, PBS Only

ALPK1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67689-1-PBS
Clone No.2B8B5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Chromosome 4 kinase, ALPK, Alpha-protein kinase 1, Alpha protein kinase 1, alpha kinase 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67689-1-PBS targets ALPK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ALPK1 fusion protein Ag5320 相同性解析による交差性が予測される生物種
Full Name alpha-kinase 1
Calculated molecular weight 139 kDa
Observed molecular weight139 kDa
GenBank accession numberBC060780
Gene Symbol ALPK1
Gene ID (NCBI) 80216
RRIDAB_2882882
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96QP1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Alpha-protein kinase 1, also known as alpha-kinase 1 (ALPK1), is associated with chronic kidney disease (CKD), myocardial infarction, gout and type 2 diabetes mellitus (DM). ALPK1 mutant has been reported to cause severe defects in motor co‐ordination in mice. ALPK1 enhances the expression of proinflammatory cytokines including IL-1β, IL-8 and TGF-β1 in cultured human THP1 and human embryonic kidney 293 (HEK293) cells (PMID: 31557402, PMID: 26275947). ALPK1 short isoform (108 kDa) was highly expressed in brain, spinal cord, heart, lung, spleen, thymus, small intestine, skin and testis, while detectable inskeletal muscle and kidney. ALPK1 large isoform (130 kDa) appeared in heart, lung, thymus and skin (PMID: 21208416).