Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse small intestine tissue, rat small intestine tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86505-1-RR targets ALPI in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg3033 Product name: Recombinant Human ALPI protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 20-528 aa of NM_001631.5 Sequence: VIPAEEENPAFWNRQAAEALDAAKKLQPIQKVAKNLILFLGDGLGVPTVTATRILKGQKNGKLGPETPLAMDRFPYLALSKTYNVDRQVPDSAATATAYLCGVKANFQTIGLSAAARFNQCNTTRGNEVISVMNRAKQAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADMPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPMGTPDPEYPADASQNGIRLDGKNLVQEWLAKHQGAWYVWNRTELMQASLDQSVTHLMGLFEPGDTKYEIHRDPTLDPSLMEMTEAALRLLSRNPRGFYLFVEGGRIDHGHHEGVAYQALTEAVMFDDAIERAGQLTSEEDTLTLVTADHSHVFSFGGYTLRGSSIFGLAPSKAQDSKAYTSILYGNGPGYVFNSGVRPDVNESESGSPDYQQQAAVPLSSETHGGEDVAVFARGPQAHLVHGVQEQSFVAHVMAFAACLEPYTACDLAPPACTTDAAHPVAASLPLLAGTLLLLGASAAP 相同性解析による交差性が予測される生物種 |
| Full Name | alkaline phosphatase, intestinal |
| Calculated molecular weight | 57 kDa |
| Observed molecular weight | 70-75 kDa |
| GenBank accession number | NM_001631.5 |
| Gene Symbol | ALPI |
| Gene ID (NCBI) | 248 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P09923 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Intestinal-type alkaline phosphatase (IAP) is one of the four human alkaline-phosphatase isoenzymes. It is synthesized by enterocytes of the small-intestinal brush border and is released into the gut lumen, blood and stool. Intestinal-type alkaline phosphatase is a constitutive, evolutionarily conserved sentinel that chemically disarms microbial toxins, orchestrates innate immunity and preserves gut homeostasis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ALPI antibody 86505-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |