Validation Data Gallery
Tested Applications
| Positive WB detected in | rat kidney tissue, mouse kidney tissue, mouse liver tissue, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33485-1-AP targets ALPI in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36025 Product name: Recombinant human ALPI protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 20-220 aa of BC132678 Sequence: VIPAEEENPAFWNRQAAEALDAAKKLQPIQKVAKNLILFLGDGLGVPTVTATRILKGQKNGKLGPETPLAMDRFPYLALSKTYNVDRQVPDSAATATAYLCGVKANFQTIGLSAAARFNQCNTTRGNEVISVMNRAKQAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADMPASARQEGCQDIATQLISNMDIDVILG 相同性解析による交差性が予測される生物種 |
| Full Name | alkaline phosphatase, intestinal |
| Calculated molecular weight | 56 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC132678 |
| Gene Symbol | ALPI |
| Gene ID (NCBI) | 248 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P09923 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ALPI (intestinal alkaline phosphatase) is a brush-border enzyme expressed in the gut that plays a critical role in maintaining intestinal homeostasis. By dephosphorylating pro-inflammatory microbial products, it helps prevent bacterial translocation and systemic inflammation. Dysfunction or deficiency of ALPI is linked to conditions such as increased intestinal permeability, metabolic disorders, and inflammatory diseases, making it a promising therapeutic target for preserving gut health.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ALPI antibody 33485-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |