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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ALMS1 Polyclonal antibody

ALMS1 Polyclonal Antibody for IF, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 27231-1-AP

Synonyms

ALMS1, ALSS, Alstrom syndrome 1, Alstrom syndrome protein 1, KIAA0328

Validation Data Gallery

Applications:
  • IF Staining of HeLa using 27231-1-AP

    IF Staining of HeLa using 27231-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 27231-1-AP (ALMS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HeLa using 27231-1-AP

    FC experiment of HeLa using 27231-1-AP

    1X10^6 HeLa cells were stained with 0.20ug ALMS1 antibody (27231-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

27231-1-AP targets ALMS1 in IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALMS1 fusion protein Ag26100 相同性解析による交差性が予測される生物種
Full Name Alstrom syndrome 1
Calculated molecular weight 461 kDa
GenBank accession numberNM_015120
Gene symbol ALMS1
Gene ID (NCBI) 7840
RRIDAB_2880812
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ALMS1 (Alstrom syndrome protein 1) is also KIAA0328. ALMS1 encodes a ~ 0.5 megadalton protein that localises to the base of centrioles. Some studies have suggested a role for this protein in maintaining centriole-nucleated sensory organelles termed primary cilia, and AS is now considered to belong to the growing class of human genetic disorders linked to ciliary dysfunction (ciliopathies). The ALMS1 protein is a component of the centrosome (PMID:30421101). ALMS1 is involved in PCM1-dependent intracellular transport. ALMS1 is required, directly or indirectly, for the localization of NCAPD2 to the proximal ends of centrioles. It is required for proper formation and/or maintenance of primary cilia (PC), microtubule-based structures that protrude from the surface of epithelial cells.

Protocols

Product Specific Protocols
IF protocol for ALMS1 antibody 27231-1-APDownload protocol
FC protocol for ALMS1 antibody 27231-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols