Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
25566-1-PBS targets ALKBH8 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22239 Product name: Recombinant human ALKBH8 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-238 aa of BC015183 Sequence: MDSNHQSNYKLSKTEKKFLRKQIKAKHTLLRHEGIETVSYATQSLVVANGGLGNGVSRNQLLPVLEKCGLVDALLMPPNKPYSFARYRTTEESKRAYVTLNGKEVVDDLGQKITLYLNFVEKVQWKELRPQALPPGLMVVEEIISSEEEKMLLESVDWTEDTDNQNSQKSLKHRRVKHFGYEFHYENNNVDKDKPLSGGLPDICESFLEKWLRKGYIKHKPDQMTINQYEPGQDCHGF 相同性解析による交差性が予測される生物種 |
| Full Name | alkB, alkylation repair homolog 8 (E. coli) |
| Calculated molecular weight | 664 aa, 75 kDa |
| GenBank accession number | BC015183 |
| Gene Symbol | ALKBH8 |
| Gene ID (NCBI) | 91801 |
| RRID | AB_3742152 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96BT7 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
ALKBH8 (Alpha-ketoglutarate-dependent dioxygenase) is unique among the ALKBH family, since it harbors a 2-oxoglutarate and Fe(II)-dependent oxygenase domain as well as an RNA recognition motif and methyl transferase domain (PMID: 27329810). ALKBH8 is a "writer" enzyme that modifies the wobble uridine of tRNA and regulates protein translation, including that of selenoproteins (PMID: 38550277). ALKBH8 is highly expressed in high-grade, superficially and deeply invasive bladder cancer. ALKBH8 knockdown induces apoptosis in bladder cancer cells (PMID: 38550277).